Application of a Colorimetric and Near-Infrared Fluorescent Probe in Peroxynitrite Detection and Imaging in Living Cells

Peroxynitrite (ONOO-) plays a vital role in pathological and physiological processes, and an excessive amount of ONOO- causes various diseases. Developing a specific and sensitive method for the detection of ONOO- in biological systems is significant. Herein, we reported a novel colorimetric and near-infrared fluorescent probe (pyridin-4-ylmethyl (Z)-2-cyano-2-(3-((E)-4-hydroxystyryl)-5,5-dimethylcyclohex-2-en-1-ylidene)acetate diphenyl phosphinate group (AN-DP)) based on isophorone and phosphinate groups for ONOO- detection. The probe displayed excellent selectivity toward ONOO- compared with other relevant analytes. It showed a good linear relationship between the fluorescence intensity at 670 nm and ONOO- concentration (0-10 mu M) with a low detection limit (53 nM). Importantly, the probe was a colorimetric and near-infrared fluorescent probe suitable for ONOO- detection. Furthermore, the probe could be used for imaging ONOO- in HepG2 cells.