Spectrometric Study on Interactions between Organochlorine Pesticides and DNA

被引：2

作者：

Lu Jia ^{[1
]}

Pu Xiao-Yang ^{[2
]}

Wang Jing ^{[1
]}

Li Hui-Hui ^{[1
]}

Yang Xiao-Di ^{[1
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat,Sch Chem & Mat Sci, Jiangsu Key Lab New Power Batteries,China Ctr Eco, Nanjing 210097, Jiangsu, Peoples R China

[2] Wannan Med Coll Pharm, Wuhu 241001, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2014年 / 42卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Organochlorine pesticides; Deoxyribonucleic acid; Interaction; Spectroscopy; Intercalation; PROTEIN; P53; INTERCALATION; RECOGNITION; DYNAMICS; ADDUCTS; SULFATE; REPAIR;

D O I：

10.3724/SP.J.1096.2014.30978

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To clarify the genotoxicity of organochlorine pesticides, the techniques of ultraviolet and visible absorption spectrum, fluorescence (FL) spectrum and circular dichroism (CD) spectrum were used to study the interaction of DDD, DDT, DDE with tumor related gene (p53 gene and C-myc gene). The experimental results of UV-vis absorption spectrum and FL spectrum indicated that the binding mode of organochlorine pesticides with DNA was mainly through intercalative mode to form the non-covalent compounds, leading to the reduction of stability of DNA with double helix. Using FL experiments, the order of binding affinity of pesticides and p53 DNA was DDE>DDT>DDD. For C-myc DNA, it was DDD>DDE>DDT. In addition, calculated thermodynamic constants confirmed that the hydrophobic action was the main interaction force. The result of CD indicated that parts of the organochlorine pesticides could affect the base stacking and secondary structure of DNA, which would further cause damage to DNA and ultimately lead to mutations.

引用

页码：482 / 488

页数：7

共 28 条

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