A sensitive enzyme-linked immunosorbent assay amplified by biotin-streptavidin system for detecting non-steroidal anti-inflammatory drug ketoprofen

被引：2

作者：

Bu, Dan ^{[1
]}

Zhuang, Hui S. ^{[1
]}

Yang, Guang X. ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Inst Environm Monitoring & Evaluat, Shanghai 200030, Peoples R China

来源：

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES | 2014年 / 49卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Biotin-streptavidin; ELISA; fish samples; ketoprofen; PPCPs; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTRON-CAPTURE DETECTOR; TANDEM MASS-SPECTROMETRY; CHLORAMPHENICOL RESIDUES; GAS-CHROMATOGRAPHY; FLORFENICOL AMINE; IMMUNOASSAY; THIAMPHENICOL; TISSUES; ELISA;

D O I：

10.1080/03601234.2014.894776

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A sensitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for detecting non-steroidal anti-inflammatory drug ketoprofen. Compared with traditional ELISA method, the sensitivity of proposed immunoassay was enhanced by the biotin-streptavidin system. Under the optimal condition, the median inhibitory concentration (IC50) was 0.25ng mL(-1), with minor cross-reactivity to a number of structural analogs. This developed assay was successfully applied to detect the ketoprofen residues in different fish samples, and good recoveries (72.6-105.5%) were obtained. The results indicated that this immunoassay method could specifically detect trace ketoprofen residues and could be widely used for routine monitoring of food samples.

引用

页码：408 / 415

页数：8

共 23 条

[1] Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey
Chen, Huaixia
Chen, Hui
Ying, Jun
Huang, Jianlin
Liao, Lei
[J]. ANALYTICA CHIMICA ACTA, 2009, 632 (01) : 80 - 85
[2] Pharmaceuticals and personal care products in the environment: Agents of subtle change?
Daughton, CG
Ternes, TA
[J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 1999, 107 : 907 - 938
[3] Ding SY, 2005, J AOAC INT, V88, P57
[4] A surface plasmon resonance biosensor assay for the simultaneous determination of thiamphenicol, florefenicol, florefenicol amine and chloramphenicol residues in shrimps
Dumont, V.
Huet, A. -C.
Traynor, I.
Elliott, C.
Delahaut, P.
[J]. ANALYTICA CHIMICA ACTA, 2006, 567 (02) : 179 - 183
[5] Fujita K, 2008, J AOAC INT, V91, P1103
[6] DEFINITION OF 3 SEROTYPES OF HANTAVIRUSES BY A DOUBLE SANDWICH ELISA WITH BIOTIN AVIDIN AMPLIFICATION SYSTEM
GOLDGABER, D
GIBBS, CJ
GAJDUSEK, DC
SVEDMYR, A
[J]. JOURNAL OF GENERAL VIROLOGY, 1985, 66 (AUG) : 1733 - 1740
[7] Guan N. P., 2007, CHIN J FOOD HYG, V19, P12
[8] Molecularly imprinted matrix solid-phase dispersion for extraction of chloramphenicol in fish tissues coupled with high-performance liquid chromatography determination
Guo, Linyuan
Guan, Min
Zhao, Chuande
Zhang, Haixia
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 392 (7-8) : 1431 - 1438
[9] Quantitative detection of tetracycline residues in honey by a simple sensitive immunoassay
Jeon, Moonsun
Paeng, Insook Rhee
[J]. ANALYTICA CHIMICA ACTA, 2008, 626 (02) : 180 - 185
[10] Biotin-avidin mediated competitive enzyme-linked immunosorbent assay to detect residues of tetracyclines in milk
Jeon, Moonsun
Kim, Jisun
Paeng, Ki-Jung
Park, Sung-Woo
Paeng, Insook Rhee
[J]. MICROCHEMICAL JOURNAL, 2008, 88 (01) : 26 - 31

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