Removal of xenotropic murine leukemia virus by nanocellulose based filter paper

被引：54
作者：
Asper, M. [1 ]
Hanrieder, T. [1 ]
Quellmalz, A. [2 ]
Mihranyan, A. [2 ]
机构：
[1] Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany
[2] Uppsala Univ, Dept Engn Sci, Nanotechnol & Funct Mat, S-75121 Uppsala, Sweden
来源：
BIOLOGICALS | 2015年 / 43卷 / 06期
关键词：
Viral contamination; Virus retentive filtration; Cladophora cellulose; Paper making; Recombinant proteins and monoclonal antibodies; VIRAL PARTICLES; CHO-CELLS; CONTAMINATION; ORGANIZATION; SEQUENCES; MEMBRANES; CELLULOSE;
D O I：
10.1016/j.biologicals.2015.08.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The removal of xenotrpic murine leukemia virus (xMuLV) by size-exclusion filter paper composed of 100% naturally derived cellulose was validated. The filter paper was produced using cellulose nanofibers derived from Cladophora sp. algae. The filter paper was characterized using atomic force microscopy, scanning electron microscopy, helium pycnometry, and model tracer (100 nm latex beads and 50 nm gold nanoparticles) retention tests. Following the filtration of xMuLV spiked solutions, LRV >= 5.25 log(10) TCID50 was observed, as limited by the virus titre in the feed solution and sensitivity of the tissue infectivity test. The results of the validation study suggest that the nanocellulose filter paper is useful for removal of endogenous rodent retroviruses and retrovirus-like particles during the production of recombinant proteins. (C) 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：452 / 456
页数：5
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