How lipid hydration and temperature affect the structure of DC-Chol-DOPE/DNA lipoplexes

被引：28

作者：

Pozzi, Daniela

Amenitsch, Heinz

Caminiti, Ruggero

Caracciolo, Giulio

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[2] Austrian Acad Sci, Inst Biophys & Xray Struct Res, Graz, Austria

来源：

CHEMICAL PHYSICS LETTERS | 2006年 / 422卷 / 4-6期

关键词：

D O I：

10.1016/j.cplett.2006.02.106

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Effect of lipid hydration on the structure of lamellar lipoplexes made of the cationic lipid 3-[N-(N,N-dimethylaminoethane)-carbamoyl]cholesterol (DC-Chol), the neutral 'helper' lipid dioleoylphosphatidylethanolamine (DOPE) and calf-thymus DNA was investigated by synchrotron small angle X-ray diffraction (SAXD). Here, we show that lipid hydration is the key factor regulating the equilibrium structure of lipoplexes. Thermotropic behavior was also investigated between 5 and 65 degrees C. Both the membrane thickness and the water layer thickness were found to decrease linearly as a function of temperature while the one dimensional DNA rod lattice between lipid bilayers was found to enlarge. Structural results were interpreted in terms of recently proposed theoretical models. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：439 / 445

页数：7

共 35 条

[1] 3β [N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol)-mediated gene delivery to primary rat neurons:: Characterization and mechanism [J].