Serial block face scanning electron microscopy-the future of cell ultrastructure imaging

被引：68

作者：

Hughes, Louise ^{[1
]}

Hawes, Chris ^{[1
]}

Monteith, Sandy ^{[2
]}

Vaughan, Sue ^{[1
]}

机构：

[1] Oxford Brookes Univ, Dept Biol & Med Sci, Oxford OX3 0BP, England

[2] Gatan UK, Abingdon OX14 1RL, Oxon, England

来源：

PROTOPLASMA | 2014年 / 251卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Serial block face scanning electron microscopy; SBEM; Selective staining; 3D reconstruction; ENDOPLASMIC-RETICULUM; CRYOELECTRON TOMOGRAPHY; MOLECULAR ARCHITECTURE; STAINING TECHNIQUE; ARRAY TOMOGRAPHY; RECONSTRUCTION; SECTIONS; IMPREGNATION; RESOLUTION; APPARATUS;

D O I：

10.1007/s00709-013-0580-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

One of the major drawbacks in transmission electron microscopy has been the production of three-dimensional views of cells and tissues. Currently, there is no one suitable 3D microscopy technique that answers all questions and serial block face scanning electron microscopy (SEM) fills the gap between 3D imaging using high-end fluorescence microscopy and the high resolution offered by electron tomography. In this review, we discuss the potential of the serial block face SEM technique for studying the three-dimensional organisation of animal, plant and microbial cells.

引用

页码：395 / 401

页数：7

共 48 条

[1] Imaging Transient Blood Vessel Fusion Events in Zebrafish by Correlative Volume Electron Microscopy [J].

Armer, Hannah E. J. ;

Mariggi, Giovanni ;

Png, Ken M. Y. ;

Genoud, Christel ;

Monteith, Alexander G. ;

Bushby, Andrew J. ;

Gerhardt, Holger ;

Collinson, Lucy M. .

PLOS ONE, 2009, 4 (11)

[2] GRAPHIC RECONSTRUCTION OF THE 3RD DIMENSION FROM SERIAL ELECTRON MICROPHOTOGRAPHS [J].

BANG, BG ;

BANG, FB .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1957, 1 (02) :138-146

[3] RECONSTRUCTION OF THE NUCLEAR SITES OF SALMONELLA-TYPHIMURIUM FROM ELECTRON MICROGRAPHS OF SERIAL SECTIONS [J].

BIRCHANDERSEN, A .

JOURNAL OF GENERAL MICROBIOLOGY, 1955, 13 (02) :327-&

[4] Volume electron microscopy for neuronal circuit reconstruction [J].

Briggman, Kevin L. ;

Bock, Davi D. .

CURRENT OPINION IN NEUROBIOLOGY, 2012, 22 (01) :154-161

[5] Wiring specificity in the direction-selectivity circuit of the retina [J].

Briggman, Kevin L. ;

Helmstaedter, Moritz ;

Denk, Winfried .

NATURE, 2011, 471 (7337) :183-U67

[6] SINGLE PARTICLE ANALYSIS AT HIGH RESOLUTION [J].

Cong, Yao ;

Ludtke, Steven J. .

METHODS IN ENZYMOLOGY, VOL 482: CRYO-EM, PART B: 3-D RECONSTRUCTION, 2010, 482 :211-235

[7] Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure [J].

Denk, W ;

Horstmann, H .

PLOS BIOLOGY, 2004, 2 (11) :1900-1909

[8] Electron tomography of ER, Golgi and related membrane systems [J].

Donohoe, Bryon S. ;

Mogelsvang, Soren ;

Staehelin, L. Andrew .

METHODS, 2006, 39 (02) :154-162

[9] Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging [J].

Ellisman, Mark H. ;

Deerinck, Thomas J. ;

Shu, Xiaokun ;

Sosinsky, Gina E. .

CORRELATIVE LIGHT AND ELECTRON MICROSCOPY, 2012, 111 :139-155

[10]

Erdman N, 2012, LOW VOLTAGE ELECT MI

← 1 2 3 4 5 →