Human organoid models to study SARS-CoV-2 infection

被引：90

作者：

Han, Yuling ^{[1
]}

Yang, Liuliu ^{[1
]}

Lacko, Lauretta A. ^{[1
]}

Chen, Shuibing ^{[1
]}

机构：

[1] Weill Cornell Med, Dept Surg, New York, NY 10065 USA

来源：

NATURE METHODS | 2022年 / 19卷 / 04期

关键词：

STEM-CELL; NEURAL PROGENITORS; IN-VITRO; CEREBRAL ORGANOIDS; VIRUS-INFECTION; CHOROID-PLEXUS; LUNG; ZIKA; DIFFERENTIATION; RESPONSES;

D O I：

10.1038/s41592-022-01453-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the deadliest pandemics in history. SARS-CoV-2 not only infects the respiratory tract, but also causes damage to many organs. Organoids, which can self-renew and recapitulate the various physiology of different organs, serve as powerful platforms to model COVID-19. In this Perspective, we overview the current effort to apply both human pluripotent stem cell-derived organoids and adult organoids to study SARS-CoV-2 tropism, host response and immune cell-mediated host damage, and perform drug discovery and vaccine development. We summarize the technologies used in organoid-based COVID-19 research, discuss the remaining challenges and provide future perspectives in the application of organoid models to study SARS-CoV-2 and future emerging viruses. This Perspective discusses the use of organoid technology, its current limitations and unmet needs in the context of SARS-CoV-2 research.

引用

页码：418 / 428

页数：11

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