GTP, an inhibitor of transglutaminases, is hydrolyzed by tissue-type transglutaminase (TGase 2) but not by epidermal-type transglutaminase (TGase 3)

被引：13

作者：

Hitomi, K ^{[1
]}

Ikura, K

Maki, M

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biosci, Nagoya, Aichi 4648601, Japan

[2] Kyoto Inst Technol, Fac Text Sci, Dept Appl Biol, Kyoto 606, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 03期

关键词：

transglutaminase; GTP; GTPase; calcium;

D O I：

10.1271/bbb.64.657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epidermal-type transglutaminase (TGase 3) is devoid of GTPase activity, but its TGase activity is inhibited by GTP as in the case of tissue-type TGase (TGase 2). In addition, the inhibition was not affected by the presence of higher concentrations of Ca ion. These results indicate that GTP interacts with TGase 3 in a manner different from its action on TGase 2.

引用

页码：657 / 659

页数：3

共 13 条

[1]

ACHYUTHAN KE, 1987, J BIOL CHEM, V262, P1901

[2]

AESCHLIMANN D, 1994, THROMB HAEMOSTASIS, V71, P402

[3]

Folk JE., 1970, METHOD ENZYMOL, V17, P889, DOI 10.1016/0076-6879(71)17302-8

[4] TRANSGLUTAMINASES - MULTIFUNCTIONAL CROSS-LINKING ENZYMES THAT STABILIZE TISSUES [J].

GREENBERG, CS ;

BIRCKBICHLER, PJ ;

RICE, RH .

FASEB JOURNAL, 1991, 5 (15) :3071-3077

[5] Characterization of recombinant mouse epidermal-type transglutaminase (TGase 3): Regulation of its activity by proteolysis and guanine nucleotides [J].

Hitomi, K ;

Kanehiro, S ;

Ikura, K ;

Maki, M .

JOURNAL OF BIOCHEMISTRY, 1999, 125 (06) :1048-1054

[6] INTERACTION SITE OF GTP-BINDING G(H) (TRANSGLUTAMINASE-II) WITH PHOSPHOLIPASE-C [J].

HWANG, KC ;

GRAY, CD ;

SIVASUBRAMANIAN, N ;

IM, MJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (45) :27058-27062

[7]

KIM HC, 1990, J BIOL CHEM, V265, P21971

[8]

KIM IG, 1993, J BIOL CHEM, V268, P12682

[9] GTP HYDROLYSIS BY GUINEA-PIG LIVER TRANSGLUTAMINASE [J].

LEE, KN ;

BIRCKBICHLER, PJ ;

PATTERSON, MK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (03) :1370-1375

[10] G(H) - A GTP-BINDING PROTEIN WITH TRANSGLUTAMINASE ACTIVITY AND RECEPTOR SIGNALING FUNCTION [J].

NAKAOKA, H ;

PEREZ, DM ;

BAEK, KJ ;

DAS, T ;

HUSAIN, A ;

MISONO, K ;

IM, MJ ;

GRAHAM, RM .

SCIENCE, 1994, 264 (5165) :1593-1596

← 1 2 →