Gene duplication event in family 12 glycosyl hydrolase from Phytophthora spp.

被引：25
作者：
Costanzo, Stefano
Ospina-Giraldo, M. D.
Deahl, K. L.
Baker, C. J.
Jones, Richard W.
机构：
[1] USDA ARS, Vegetable Lab, Beltsville, MD 20705 USA
[2] Univ Maryland, Dept Nat Resource Sci & Landscape Architecture, College Pk, MD 20742 USA
[3] Lafayette Coll, Dept Biol, Easton, PA 18042 USA
[4] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA
来源：
FUNGAL GENETICS AND BIOLOGY | 2006年 / 43卷 / 10期
关键词：
xyloglucan hydrolase; endoglucanase; Phytophthora infestans; Phytophthora sojae; Phytophthora ramorum;
D O I：
10.1016/j.fgb.2006.04.006
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A total of 18 paralogs of xyloglucan-specific endoglucanases (EGLs) from the glycosyl hydrolase family 12 were identified and characterized in Phytophthora sojae and Phytophthora ramorum. These genes encode predicted extracellular enzymes, with sizes ranging from 189 to 435 amino acid residues, that would be capable of hydrolyzing the xyloglucan component of the host cell wall. In two cases, four and six functional copies of these genes were found in tight succession within a region of 5 and 18 kb, respectively. The overall gene copy number and relative organization appeared well conserved between P. sojae and P. ramorum, with apparent synteny in this region of their respective genomes. Phylogenetic analyses of Phytophthora endoglucanases of family 12 and other known members of EGL 12, revealed a close relatedness with a fairly conserved gene sub-family containing, among others, sequences from the fungi Emericella desertorum and Aspergillus aculeatus. This is the first report of family 12 EGLs present in plant pathogenic eukaryotes. Published by Elsevier Inc.
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页码：707 / 714
页数：8
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