Time-resolved fluorescence immunoassay of thyroxine in serum: Immobilized antigen approach

被引:22
作者
Wu, FB [1 ]
Xu, YY [1 ]
Xu, T [1 ]
Wang, YZ [1 ]
Han, SQ [1 ]
机构
[1] China Inst Atom Energy, Dept Isotope, Lab Immunoassay, Beijing 102413, Peoples R China
关键词
D O I
10.1006/abio.1999.4342
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
With T-4-bovine IgG as a solid-phase antigen, we have developed a direct competitive-type immunoassay for serum total thyroxine (TT4), which depends on the competitive distribution of europium-labeled anti-T-4 monoclonal antibody between solid-phase-bound T-4 and the T-4 in the sample or standard. The captured fraction of the tracer was measured after a dissociation-enhancement step. Four different T-4 protein conjugates were synthesized, of which T-4-bovine IgG was selected as the most favorable for the preparation of solid-phase antigen. The sensitivity was 3.5 ng/ml with a sample volume of 20 mu I. T-4 values obtained by this procedure agreed well with those obtained by RIA (r = 0.967, n = 38) and EG&G Wallac TRFIA. (r = 0.926, n = 64). All other quality criteria was also fulfilled with respect to precision, accuracy, and dynamic range. (C) 1999 Academic Press.
引用
收藏
页码:171 / 176
页数:6
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