Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line

被引：22
作者：
Freitas, VM
Scheremeta, B
Hoffman, MP
Jaeger, RG
机构：
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Histol & Embryol, BR-05508900 Sao Paulo, Brazil
[2] Penn State Univ, University Pk, PA 16802 USA
[3] NIDCR, CDBRB, NIH, Bethesda, MD USA
来源：
ORAL ONCOLOGY | 2004年 / 40卷 / 05期
基金：
巴西圣保罗研究基金会;
关键词：
salivary gland neoplasms; adenoid cystic carcinoma; extracellular matrix; laminin; matrix metalloproteinases;
D O I：
10.1016/j.oraloncology.2003.10.002
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/ activating rote of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：483 / 489
页数：7
相关论文
共 30 条
  • [11] The basement membrane matrix in malignancy
    Engbring, JA
    Kleinman, HK
    [J]. JOURNAL OF PATHOLOGY, 2003, 200 (04) : 465 - 470
  • [12] The role of basement membrane proteins on the expression of neural cell adhesion molecule (N-CAM) in an adenoid cystic carcinoma cell line
    França, CM
    Jaeger, MMM
    Jaeger, RG
    Araújo, NS
    [J]. ORAL ONCOLOGY, 2000, 36 (02): : 248 - 252
  • [13] Effect of N-CAM on in vitro invasion of human adenoid cystic carcinoma cells
    França, CM
    Jaeger, RG
    Freitas, VM
    Araújo, NS
    Jaeger, MMM
    [J]. ORAL ONCOLOGY, 2001, 37 (08) : 638 - 642
  • [14] The effect of laminin and its peptide SIKVAV on a human salivary gland adenoid cystic carcinoma cell line
    Freitas, VM
    Jaeger, RG
    [J]. VIRCHOWS ARCHIV, 2002, 441 (06) : 569 - 576
  • [15] Reconstruction of pleomorphic adenoma of the salivary glands in three-dimensional collagen gel matrix culture
    Fujita, Y
    Yoshida, T
    Sakakura, A
    Sakakura, T
    [J]. VIRCHOWS ARCHIV, 1999, 434 (02) : 137 - 143
  • [16] Effect of spatial arrangement of the basement membrane on cultured pleomorphic adenoma cells. Study by immunocytochemistry and electron and confocal microscopy
    Jaeger, MMM
    Araujo, VC
    Kachar, B
    Jaeger, RG
    [J]. VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY, 1997, 430 (06): : 467 - 477
  • [17] Expression of smooth-muscle actin in cultured cells from human plasmacytoid myoepithelioma
    Jaeger, RG
    de Oliveira, PT
    Jaeger, MMM
    de Araujo, VC
    [J]. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1997, 84 (06): : 663 - 667
  • [18] ROLE OF THE SIKVAV SITE OF LAMININ IN PROMOTION OF ANGIOGENESIS AND TUMOR-GROWTH - AN INVIVO MATRIGEL MODEL
    KIBBEY, MC
    GRANT, DS
    KLEINMAN, HK
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1992, 84 (21) : 1633 - 1638
  • [19] BASEMENT-MEMBRANE COMPLEXES WITH BIOLOGICAL-ACTIVITY
    KLEINMAN, HK
    MCGARVEY, ML
    HASSELL, JR
    STAR, VL
    CANNON, FB
    LAURIE, GW
    MARTIN, GR
    [J]. BIOCHEMISTRY, 1986, 25 (02) : 312 - 318
  • [20] LIOTTA LA, 1986, CANCER RES, V46, P1
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