Simultaneous Separation, Metering, and Dilution of Plasma from Human Whole Blood in a Microfluidic System

被引：73

作者：

Tachi, Tomoya ^{[1
]}

Kaji, Noritada ^{[1
,2
]}

Tokeshi, Manabu ^{[1
,2
]}

Baba, Yoshinobu ^{[1
,2
,3
,4
,5
]}

机构：

[1] Nagoya Univ, Dept Appl Chem, Grad Sch Engn, Nagoya, Aichi 4648603, Japan

[2] Nagoya Univ, MEXT Innovat Res Ctr Prevent Med Engn, Nagoya, Aichi 4648603, Japan

[3] Nagoya Univ, Plasma Nanotechnol Res Ctr, Nagoya, Aichi 4648603, Japan

[4] Natl Inst Adv Ind Sci & Technol, Hlth Technol Res Ctr, Takamatsu, Kagawa 7610395, Japan

[5] Natl Inst Nat Sci, Inst Mol Sci, Okazaki, Aichi 4448585, Japan

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 08期

关键词：

MICROCHIP CAPILLARY-ELECTROPHORESIS; ON-A-CHIP; CROSS-FLOW; LITHIUM; DEVICE;

D O I：

10.1021/ac802434z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In about a 3 min period, we have simultaneously separated plasma from human whole blood and metered and diluted the plasma using a microchip with an interchannel microstructure. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting operations of the plasma were based on volume control of liquid in the microchannels by syringe pumps. On this microchip, we produced plasma diluted by a factor of 6 from whole blood containing theophylline and we observed very little hemolysis. It is possible to separate plasma from one or just several drops of whole blood by using this microchip.

引用

页码：3194 / 3198

页数：5

共 19 条

[1] Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications [J].

Crowley, TA ;

Pizziconi, V .

LAB ON A CHIP, 2005, 5 (09) :922-929

[2] A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability [J].

Easley, Christopher J. ;

Karlinsey, James M. ;

Bienvenue, Joan M. ;

Legendre, Lindsay A. ;

Roper, Michael G. ;

Feldman, Sanford H. ;

Hughes, Molly A. ;

Hewlett, Erik L. ;

Merkel, Tod J. ;

Ferrance, Jerome P. ;

Landers, James P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (51) :19272-19277

[3] Sensitivity enhancement for colorimetric glucose assays on whole blood by on-chip beam-guidance [J].

Grumann, M. ;

Steigert, J. ;

Riegger, L. ;

Moser, I. ;

Enderle, B. ;

Riebeseel, K. ;

Urban, G. ;

Zengerle, R. ;

Ducree, J. .

BIOMEDICAL MICRODEVICES, 2006, 8 (03) :209-214

[4] In situ fabricated porous filters for microsystems [J].

Moorthy, J ;

Beebe, DJ .

LAB ON A CHIP, 2003, 3 (02) :62-66

[5] Continuous separation of lipid particles from erythrocytes by means of laminar flow and acoustic standing wave forces [J].

Petersson, F ;

Nilsson, A ;

Holm, C ;

Jönsson, H ;

Laurell, T .

LAB ON A CHIP, 2005, 5 (01) :20-22

[6] Microfluidic tool box as technology platform for hand-held diagnostics [J].

Pugia, MJ ;

Blankenstein, G ;

Peters, RP ;

Profitt, JA ;

Kadel, K ;

Willms, T ;

Sommer, R ;

Kuo, HH ;

Schulman, LS .

CLINICAL CHEMISTRY, 2005, 51 (10) :1923-1932

[7] A method for correcting the erythrocyte sedimentation rate for variations in the cell volume percentage of blood [J].

Rourke, MD ;

Ernstene, AC .

JOURNAL OF CLINICAL INVESTIGATION, 1930, 8 (04) :545-559

[8] A hybrid poly(dimethylsiloxane) microsystem for on-chip whole blood filtration optimized for steroid screening [J].

Thorslund, S ;

Klett, O ;

Nikolajeff, F ;

Markides, K ;

Bergquist, J .

BIOMEDICAL MICRODEVICES, 2006, 8 (01) :73-79

[9] Blood-on-a-chip [J].

Toner, M ;

Irimia, D .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2005, 7 :77-103

[10] Perfusion in microfluidic cross-flow: Separation of white blood cells from whole blood and exchange of medium in a continuous flow [J].

VanDelinder, Virginia ;

Groisman, Alex .

ANALYTICAL CHEMISTRY, 2007, 79 (05) :2023-2030

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