Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model

被引:20
作者
Amorim, Jaime Henrique [1 ]
dos Santos Alves, Rubens Prince [1 ]
Bizerra, Raiza [1 ]
Pereira, Sara Araujo [1 ]
Pereira, Lennon Ramos [1 ]
Nascimento Fabris, Denicar Lina [1 ]
Santos, Robert Andreata [1 ]
Romano, Camila Malta [2 ,3 ]
de Souza Ferreira, Luis Carlos [1 ]
机构
[1] Univ Sao Paulo, Dept Microbiol, Lab Desenvolvimento Vacinas, BR-05508000 Sao Paulo, SP, Brazil
[2] Univ Sao Paulo, Dept Mol Infecciosas & Parasitarias LIMHC, Inst Med Trop Sao Paulo, BR-05508000 Sao Paulo, SP, Brazil
[3] Univ Sao Paulo, Fac Med, BR-05508000 Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Dengue; Nonstructural proteins; Immune response; T lymphocytes; Antibodies; CD8(+) T-CELL; WEST-NILE-VIRUS; NEUTRALIZING ANTIBODY; VACCINE FORMULATIONS; GAMMA-INTERFERON; DOMAIN-III; LYMPHOCYTES; RECOMBINANT; CD4(+); MICE;
D O I
10.1016/j.virol.2015.10.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Generating neutralizing antibodies have been considered a prerequisite to control dengue virus (DENV) infection. However, T lymphocytes have also been shown to be important in a protective immune state. In order to investigate the contribution of both humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to intracranially prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8(+) T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to naive mice challenged with the JHA1 strain. In contrast, depletion of CD4(+) and CD8(+) T lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:41 / 49
页数:9
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