Electrochemical laccase sensor based on 3-methylthiophene/3-thiopheneacetic acid/bis(3,4-ethylenedioxythiophene)-N-nonylacridone as a new polymer support

被引:9
作者
Jedrychowska, Agnieszka [1 ]
Cabaj, Joanna [1 ]
Swist, Agnieszka [1 ]
Soloducho, Jadwiga [1 ]
机构
[1] Wroclaw Univ Technol, Dept Med Chem & Microbiol, Fac Chem, PL-50370 Wroclaw, Poland
关键词
Conducting copolymer; Laccase; Cerrena unicolor; Enzymatic electrode; Sensor; DIRECT ELECTRON-TRANSFER; TRAMETES-VERSICOLOR; CONDUCTING POLYMERS; IMMOBILIZATION; ENZYMES;
D O I
10.1016/j.jelechem.2014.03.017
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An amperometric biosensing electrode was constructed by immobilization of laccase in an electrochemically synthesized copolymer based on N-alkylacridone derivative on a Pt electrode. Laccase from Cerrena unicolor has been successfully immobilized (electrolytic deposition) on the surface of thin film built of 3-methylthiophene/3-thiopheneacetic acid/2,7-bis[2-(3,4-etylenedioxythiophene)]-N-nonylacridone. A clearly defined reduction current according to the phenolic compound presence was observed in cyclic voltammetry, which attributed to the reduction of enzymatically produced quinone on the electrode surface. The immersion of the laccase-coated electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry at low potential scan rates. The response of the biosensing platform is evaluated in the presence of hydroquinone (HQ) and syringaldazine, used as phenolic enzymatic substrates. The system exhibits a clear electrocatalytic activity and the substrates can be amperometrically determined at -0.10 V vs. SCE. Considering the fact, that the immobilization strategy showed high efficiency, obtained results suggest that the method for phenoloxidase immobilization has a great potential of enabling high throughput fabrication of bioelectronic devices. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:64 / 70
页数:7
相关论文
共 39 条
[1]   Biomolecular immobilization on conducting polymers for biosensing applications [J].
Ahuja, Tarushee ;
Mir, Irfan Ahmad ;
Kumar, Devendra ;
Rajesh .
BIOMATERIALS, 2007, 28 (05) :791-805
[2]   Immobilization of wood-rotting fungi laccases on modified cellulose and acrylic carriers [J].
Al-Adhami, AJH ;
Bryjak, J ;
Greb-Markiewicz, B ;
Peczynska-Czoch, W .
PROCESS BIOCHEMISTRY, 2002, 37 (12) :1387-1394
[3]  
[Anonymous], 2001, ELECTROCHEMICAL METH
[4]   A stable electrode for high-potential, electrocatalytic O2 reduction based on rational attachment of a blue copper oxidase to a graphite surface [J].
Blanford, Christopher F. ;
Heath, Rachel S. ;
Armstrong, Fraser A. .
CHEMICAL COMMUNICATIONS, 2007, (17) :1710-1712
[5]   Peculiarities of direct bioelectrocatalysis by laccase in aqueous-nonaqueous mixtures [J].
Bogdanovskaya, VA ;
Tarasevich, MR ;
Kuznetsova, LN ;
Reznik, MF ;
Kasatkin, EV .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (11-12) :945-951
[6]   A novel electrochemical synthesis of poly-3-methylthiophene-γ-cyclodextrin film -: Application for the analysis of chlorpromazine and some neurotransmitters [J].
Bouchta, D ;
Izaoumen, N ;
Zejli, H ;
El Kaoutit, M ;
Temsamani, KR .
BIOSENSORS & BIOELECTRONICS, 2005, 20 (11) :2228-2235
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   PEI-coated gold nanoparticles decorated with laccase: A new platform for direct electrochemistry of enzymes and biosensing applications [J].
Brondani, Daniela ;
de Souza, Bernardo ;
Souza, Bruno S. ;
Neves, Ademir ;
Vieira, Iolanda C. .
BIOSENSORS & BIOELECTRONICS, 2013, 42 :242-247
[9]   Oxygen transport through laccase biocathodes for a membrane-less glucose/O2 biofuel cell [J].
Brunel, L. ;
Denele, J. ;
Servat, K. ;
Kokoh, K. B. ;
Jolivalt, C. ;
Innocent, C. ;
Cretin, M. ;
Rolland, M. ;
Tingry, S. .
ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (02) :331-336
[10]   Hybrid phenol biosensor based on modified phenoloxidase electrode [J].
Cabaj, Joanna ;
Soloducho, Jadwiga ;
Chyla, Antoni ;
Jedrychowska, Agnieszka .
SENSORS AND ACTUATORS B-CHEMICAL, 2011, 157 (01) :225-231