Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation

被引：16

作者：

Luz, Valesca B. ^{[1
]}

Santos, Regiane R. ^{[2
]}

Pinto, Leonardo C. ^{[1
]}

Soares, Alison A. X. ^{[1
]}

Celestino, Juliana J. H. ^{[1
]}

Mafezoli, Jair ^{[3
]}

Campello, Claudio C. ^{[1
]}

Figueiredo, Jose R. ^{[1
]}

Rodrigues, Ana P. R. ^{[1
]}

机构：

[1] Univ Ceara, Fac Vet Med, Lab Manipulat Oocytes Enclosed Preantral Follicle, Fortaleza, Ceara, Brazil

[2] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, Utrecht, Netherlands

[3] Univ Fortaleza, Dept Life Sci, Fortaleza, Ceara, Brazil

来源：

FERTILITY AND STERILITY | 2009年 / 91卷 / 04期

关键词：

DMSO; cryopreservation; HPLC;

D O I：

10.1016/j.fertnstert.2008.07.1778

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Ovarian cortical fragments (3 x 3 x 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure. (Fertil Steril (R) 2009;91:1513-5. (C)2009 by American Society for Reproductive Medicine.)

引用

页码：1513 / 1515

页数：3

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