Diversity and persistence of Listeria monocytogenes within the Gorgonzola PDO production chain and comparison with clinical isolates from the same area

被引:13
作者
Filipello, Virginia [1 ]
Gallina, Silvia [2 ]
Amato, Ettore [3 ]
Losio, Marina Nadia [4 ]
Pontello, Mirella [3 ]
Decastelli, Lucia [2 ]
Lomonaco, Sara [1 ]
机构
[1] Univ Turin, Dept Vet Sci, Largo P Braccini 2, D-10095 Grugliasco, Italy
[2] Ist Zooprofilattico Sperimentale Piemonte Liguria, Via Bologna 48, I-10154 Turin, Italy
[3] Univ Milan, Dept Hlth Sci, Via Rudini 8, I-20142 Milan, Italy
[4] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Via Bianchi 9, I-25124 Brescia, Italy
关键词
Listeria monocytogenes; Food-bome disease; Epidemic clones; Multi-virulence-locus sequence typing; Persistence; Dairy; EPIDEMIC CLONES; PASTEURIZED MILK; OUTBREAK STRAINS; UNITED-STATES; CHEESE; VIRULENCE; GASTROENTERITIS; SEROTYPES; CANADA;
D O I
10.1016/j.ijfoodmicro.2017.01.012
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Listeria monocytogenes causes invasive syndromes with high fatality rates in specific population groups. Cheeses have been commonly implicated in outbreaks worldwide. Gorgonzola is a cheese only produced in Northwestern Italy (it is the third Italian cheese in terms of production and export) and L. monocytogenes is frequently isolated from the production chain. The aims of this study were to assess the distribution of L monocytogenes Virulence Types (VTs) in isolates collected in Gorgonzola processing plants and to determine the presence of Epidemic Clones (ECs). Fifty-Six L. monocytogenes strains collected between 2004 and 2016 from cheese and environmental samples were subtyped with Multi-Virulence-Locus Sequence Typing (MVLST) and compared to previously typed strains. Most isolates (eta = 50) belonged to two new VTs (Vr113 arid VT114). The remaining isolates belonged to previously identified VTs: VT14-ECVIII (milk chocolate outbreak, 1994, USA) and VT80 (ricotta salata outbreak, 2012, USA). VT14, VT80 and VT113 were shared with isolates from apparently sporadic human cases in the same geographical area and temporal period (Piedmont and Lombardy, 2005-2016). The overall L. monocytogenes population appears to be homogeneous and may be characteristic of Gorgonzola production. Nevertheless, the detection in cheese and environmental samples of VTs observed in clinical isolates or outbreak related strains (VT80, VT14) contributed to better describe the current scenario and pointed out the need for increased surveillance. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:73 / 78
页数:6
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