Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p

被引：10
作者：
Singh, Vikash [1 ]
Azad, Gajendra Kumar [1 ]
Sariki, Santhosh Kumar [1 ]
Tomar, Raghuvir S. [1 ]
机构：
[1] Indian Inst Sci Educ & Res, Dept Biol Sci, Lab Chromatin Biol, Bhopal 462023, India
来源：
FEBS LETTERS | 2015年 / 589卷 / 20期
关键词：
Flocculation; Gene regulation; Sen1; RNA helicase; RNA-SPLICING ENDONUCLEASE; TRANSCRIPTION TERMINATION; CELL-SURFACE; YEAST; GENE; PROTEIN; COMPLEX; FLO1; SENATAXIN; NRD1;
D O I：
10.1016/j.febslet.2015.09.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Nrd1-Nab3-Sen1 (NNS) complex terminates transcription of non-coding RNA genes and mediates degradation of the produced transcript by the nuclear exosome. The NNS complex also represses some stress response genes, by stimulating premature termination. A well-characterized stress response in yeast is flocculation, where cells aggregate to form flocs under expression of lectin-encoding genes designated as FLOs. In this study, we demonstrated the role of the NNS complex and Rrp6p in the expression of flocculation genes: FLO1, FLO5, FLO9, and FLOW. Furthermore, a deletion mutant of the RNA processing machinery (RNT1), and SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype. In summary, we have identified a cooperative role of Rnt1p, Rrp6p and the NNS complex in the repression of FLO genes. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3165 / 3174
页数：10
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