Antiviral immune responses in Itk-deficient mice

被引:40
作者
Bachmann, MF
Littman, DR
Liao, XC
机构
[1] UNIV ZURICH,DEPT PATHOL,CH-8006 ZURICH,SWITZERLAND
[2] UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,DEPT IMMUNOL & MICROBIOL,SAN FRANCISCO,CA 94143
关键词
D O I
10.1128/JVI.71.10.7253-7257.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mice lacking Itk, a T-cell-specific protein tyrosine kinase, have reduced numbers of T cells and reduced responses to allogeneic major histocompatibility molecules, This study analyzed antiviral immune responses in mice deficient for Itk, Primary cytotoxic T-lymphocyte (CTL) responses were analyzed after infection with lymphocytic choriomeningitis virus (LCMV), vaccinia virus (VV), and vesicular stomatitis virus (VSV), Ex vivo CTL activity was consistently reduced by a factor of two to six for the different viruses, CTL responses after restimulation in vitro were similarly reduced unless exogenous cytokines were added, In the presence of interleukin-2 or concanavalin A supernatant, Itk deficient and control mice responded similarly, Interestingly, while LCMV was completely eliminated by day 8 in both Itk-deficient and control mice, VV cleared from itk(-/-) mice with delayed kinetics. Antibody responses were evaluated after VSV infection, Both the T-cell-independent neutralizing immunoglobulin M (IgM) and the T-cell-dependent IgG responses were similar in Itk-deficient and control mice, Taken together, the results show that CTL responses are reduced in the absence of Itk whereas antiviral B-cell responses are not affected.
引用
收藏
页码:7253 / 7257
页数:5
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