Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme

被引：7

作者：

Qu, Jiagui ^{[1
,2
]}

Rizak, Joshua D. ^{[2
,3
]}

Fan, Yaodong ^{[4
]}

Guo, Xiaoxuan ^{[5
]}

Li, Jiejing ^{[6
]}

Huma, Tanzeel ^{[2
,7
]}

Ma, Yuanye ^{[2
]}

机构：

[1] Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China

[2] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Brain & Cognit Sci, Kunming 650223, Yunnan, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100101, Peoples R China

[4] Kunming Med Univ, Affiliated Hosp 3, Dept Neurosurg, Yunnan Prov Tumor Hosp, Kunming 650118, Yunnan, Peoples R China

[5] China Agr Univ, Beijing Key Lab Funct Food Plant Resources, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[6] Chinese Acad Sci, Kunming Inst Zool, CAS Max Planck Jr Scientist Grp, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China

[7] Quaid I Azam Univ, Fac Biol Sci, Dept Anim Sci, Reprod Neuroendocrinol Lab, Islamabad 45320, Pakistan

来源：

HUMAN CELL | 2014年 / 27卷 / 03期

关键词：

Cell line; Glioblastoma multiforme; Left prefrontal lobe; Gene transcription; BETA-TUBULIN ISOTYPE; EMBRYONIC STEM-CELLS; MALIGNANT GLIOMA; BRAIN-TUMORS; NESTIN; EXPRESSION; PROTEIN; CANCER; COEXPRESSION; PHENOTYPE;

D O I：

10.1007/s13577-013-0086-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This paper outlines the establishment of a new and stable cell line, designated GBM-HSF, from a malignant glioblastoma multiforme (GBM) removed from a 65-year-old Chinese woman. This cell line has been grown for 1 year without disruption and has been passaged over 50 times. The cells were adherently cultured in RPMI-1640 media with 10 % fetal bovine serum supplementation. Cells displayed spindle and polygonal morphology, and displayed multi-layered growth without evidence of contact inhibition. The cell line had a high growth rate with a doubling time of 51 h. The cells were able to grow without adhering to the culture plates, and 4.5 % of the total cells formed colonies in soft agar. The cell line has also been found to form tumors in nude mice and to be of a highly invasive nature. The cells were also partially characterized with RT-PCR. The RT-PCR revealed that Nestin, beta-tubulin III, Map2, Klf4, Oct4, Sox2, Nanog, and CD26 were positively transcribed, whereas GFAP, Rex1, and CD133 were negatively transcribed in this cell line. These results suggest that the GBM-HSF cell line will provide a good model to study the properties of cancer stem cells and metastasis. It will also facilitate more detailed molecular and cellular studies of GBM cell division and pathology.

引用

页码：129 / 136

页数：8

共 29 条

[1] Rex-1, a gene encoding a transcription factor expressed in rbe early embryo, is regulated via Oct-3/4 and Oct-6 binding to an Octamer site and a novel protein, Rox-1, binding to an adjacent site [J].