C to U editing of the anticodon of imported mitochondrial tRNATrp allows decoding of the UGA stop codon in Leishmania tarentolae

被引：109

作者：

Alfonzo, JD

Blanc, V

Estévez, AM

Rubio, MAT

Simpson, L ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Dept Med Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

来源：

EMBO JOURNAL | 1999年 / 18卷 / 24期

关键词：

anticodon; editing; mitochondria; translation; tRNA;

D O I：

10.1093/emboj/18.24.7056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All mitochondrial tRNAs in kinetoplastid protists are encoded in the nucleus and imported into the organelle, The tRNA(Trp)(CCA) can decode the standard UGG tryptophan codon but can not decode the mitochondrial UGA tryptophan codon, We show that the mitochondrial tRNA(Trp) undergoes a specific C to U nucleotide modification in the first position of the anticodon, which allows decoding of mitochondrial UGA codons as tryptophan. Functional evidence for the absence of a UGA suppressor tRNA in the cytosol, using a reporter gene, was also obtained, which is consistent with a mitochondrial localization of this editing event. Leishmania cells have dealt with the problem of a lack of expression within the organelle of this non-universal tRNA by compartmentalizing an editing activity that modifies the anticodon of the imported tRNA.

引用

页码：7056 / 7062

页数：7