Autoinducer-2-like activity on vegetable produce and its potential involvement in bacterial biofilm formation on tomatoes

被引:38
作者
Lu, Lingeng
Hume, Michael E.
Pillai, Suresh D.
机构
[1] Texas A&M Univ, Dept Poultry Sci, Kleberg Ctr, College Stn, TX 77843 USA
[2] ARS, USDA, So Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX USA
关键词
D O I
10.1089/fpd.2005.2.242
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Quorum sensing employing autoinducer molecules is a strategy used by bacterial populations to coordinately modulate their response to environmental stresses and host defense mechanisms. The objectives of this study were to determine the levels of autoinducer-2 (AI-2)-like activity on selected vegetable produce and determine whether AI-2-like molecules can promote E. coli O157:H7 biofilm formation on tomatoes. Twelve different fruit and vegetable produce samples were screened for AI-2-like activity using autoinducer sensing V. harveyi biosensor strains. All samples except strawberries showed AI-2 activity albeit at varying levels, with eggplant having the highest levels. Tomatoes, when stored at VC for 9 days, showed increasing levels of heterotrophic bacterial populations as compared to AI-2-like activity levels, which fluctuated. Rinses from Roma tomato surfaces that were stored at refrigeration temperature for up to 9 days caused a significant increase (1.8-3.6-fold as compared to the negative controls) in biofilm formation by luxS mutant (non AI-2 producing) generic E. coli and E. coli O157:H strains using a micro-titer plate-based biofilm assay. These results suggest that AI-2-like activity, which is present on the surfaces of tomatoes, has the potential to enhance the production of bacterial biofilms.
引用
收藏
页码:242 / 249
页数:8
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