Evaluation of real-time PCR data

被引：2

作者：

Vaerman, JL ^{[1
]}

Saussoy, P ^{[1
]}

Ingargiola, I ^{[1
]}

机构：

[1] Clin Univ St Luc, Ecole Sante Publ, Mol Biol Lab, UCL, B-1200 Brussels, Belgium

来源：

JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS | 2004年 / 18卷 / 02期

关键词：

PCR; real-time PCR; validation; quantification; amplification;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

If real-time PCR is to be of much worth to its user, some idea regarding the reliability of its data is essential. We discuss here some of the problems associated with interpreting numerical real-time PCR data that lend themselves to analytical evaluation. We translate into the language of molecular biology some of the criteria which are used to evaluate the performance of any new method (linearity, precision, specificity, limit of detection and quantification).

引用

页码：212 / 214

页数：3

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