Adventitious shoot regeneration and micropropagation in Calendula officinalis L.

被引：27

作者：

Çöçü, S

Uranbey, S

Ipek, A

Khawar, KM

Sarihan, EO

Kaya, MD

Parmaksiz, I

Özcan, S

机构：

[1] Ankara Univ, Fac Agr, Dept Field Crops, TR-06110 Ankara, Turkey

[2] Minist Agr & Rural Affairs, Cent Field Crops Res Inst, TR-06170 Ankara, Turkey

来源：

BIOLOGIA PLANTARUM | 2004年 / 48卷 / 03期

关键词：

clonal propagation; cotyledon; cotyledonary node; hypocotyl; indole-3-butyric acid; kinetin; alpha-naphthalene-acetic acid; organogenesis; thidiazuron;

D O I：

10.1023/B:BIOP.0000041102.79647.b6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis L were cultured on Murashige and Skoog ( MS) media supplemented with various concentrations of thidiazuron (TDZ), kinetin ( KIN), alpha-naphthaleneacetic acid (NAA) and indole-3-butyric acid ( IBA) to induce adventitious shoot regeneration and micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg dm(-3) TDZ and either 0.25 or 0.50 mg dm(-3) IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg dm(-3) TDZ and 0.05 mg dm(-3) NAA or 2 mg dm(-3) KIN and 1 mg dm(-3) NAA. Regenerated shoots were excised and rooted in MS medium supplemented with 1 mg dm(-3) NAA. The rooted plantlets were finally transferred to pots.

引用

页码：449 / 451

页数：3

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