Absence of bovine leukosis virus in semen of seropositive bulls

被引：13
作者：
Choi, KY [1 ]
Monke, D
Stott, JL
机构：
[1] Investigen Inc, Alameda, CA USA
[2] Select Sires, Plain City, OH USA
[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2002年 / 14卷 / 05期
关键词：
D O I：
10.1177/104063870201400507
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A polymerase chain reaction (PCR)-based detection system was established to identify the presence of bovine leukosis virus (BLV) DNA in bovine semen. Seventy-nine bulls were included in the study. Serum, peripheral blood leukocytes, and semen were collected from each of the 79 bulls. The BLV-specific antibody was detected in serum by agar gel immunodiffusion and viral DNA in blood and semen by PCR. Serologically, 29 of the 79 bulls were BLV positive. Twenty-seven of the 29 seropositive bulls and I of the seronegative bulls had BLV DNA in peripheral blood leukocytes. All 79 bulls tested PCR negative for the presence of BLV in semen. This data is strong evidence that properly collected semen from BLV seropositive bulls will not contribute to dissemination of this viral infection.
引用
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页码：403 / 406
页数：4
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