Characterization of Kenyan sweet potato genotypes for SPVD resistance and high dry matter content using single sequence repeat markers

被引:0
作者
Karuri, H. W. [1 ]
Ateka, E. M. [1 ]
Amata, R. [2 ]
Nyende, A. B. [1 ]
Muigai, A. W. T. [1 ]
机构
[1] Jomo Kenyatta Univ Agr & Technol, Nairobi 00200, Kenya
[2] Kenya Agr Res Inst, Nairobi 00800, Kenya
来源
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 10期
关键词
Polymorphic information content; SPVD; severity; cluster analysis; BATATAS L. LAM; VIRUS-DISEASE; GENETIC DIVERSITY; MICROSATELLITE MARKERS; RAPD MARKERS; IPOMOEA; CULTIVARS; AFLP; DNA; TAIWAN;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Simple sequence repeat (SSR) markers were used to characterize Kenyan sweet potato genotypes for resistance to the sweet potato virus disease (SPVD) and high dry matter content. Eighty nine (89) genotypes with a mean symptom severity score of between 1 and 1.5 were selected following graft inoculation with SPVD-infected scions and characterized using 6 SSR primers. The 6 SSR primer pairs had an average polymorphic information content (PIC) of 0.47. The average number of alleles within the 89 genotypes across the 6 loci was 13.52. Cluster analyses revealed a 50% variation among the 89 genotypes. The dendrogram did not reveal any unique clustering of the genotypes according to dry matter content and reaction to SPVD. The genetic differences among the SPVD resistant genotypes and those with high dry matter revealed by the distinct groups suggest a significant genetic variability and the presence of different sources of resistance to SPVD and high dry matter. This characterization will give valuable information for breeders and serve as a baseline for efficient development of new cultivars resistant to SPVD and containing high dry matter.
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收藏
页码:2169 / 2175
页数:7
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