Progress in Quantitative Viral Load Testing: Variability and Impact of the WHO Quantitative International Standards

被引：68

作者：

Hayden, R. T. ^{[1
]}

Sun, Y. ^{[2
]}

Tang, L. ^{[2
]}

Procop, G. W. ^{[3
]}

Hillyard, D. R. ^{[4
]}

Pinsky, B. A. ^{[5
,6
]}

Young, S. A. ^{[7
,8
]}

Caliendo, A. M. ^{[9
]}

机构：

[1] St Jude Childrens Res Hosp, Dept Pathol, 332 N Lauderdale St, Memphis, TN 38105 USA

[2] St Jude Childrens Res Hosp, Dept Biostat, 332 N Lauderdale St, Memphis, TN 38105 USA

[3] Cleveland Clin, Dept Lab Med, Cleveland, OH 44106 USA

[4] Univ Utah, Sch Med, Dept Physiol, Salt Lake City, UT 84108 USA

[5] Stanford Univ, Dept Pathol, Sch Med, Stanford, CA 94305 USA

[6] Stanford Univ, Dept Med, Sch Med, Stanford, CA 94305 USA

[7] Univ New Mexico, Sch Med, Dept Pathol, Albuquerque, NM 87131 USA

[8] TriCore Reference Labs, Albuquerque, NM USA

[9] Brown Univ, Dept Med, Alpert Med Sch, Providence, RI 02912 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2017年 / 55卷 / 02期

关键词：

PCR; viral load; variability; quantitative; calibration; WHO; REAL-TIME PCR; COMMUTABILITY; VIRUS;

D O I：

10.1128/JCM.02044-16

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It has been hoped that the recent availability of WHO quantitative standards would improve interlaboratory agreement for viral load testing; however, insufficient data are available to evaluate whether this has been the case. Results from 554 laboratories participating in proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstein-Barr virus (EBV), BK virus (BKV), adenovirus (ADV), and human herpesvirus 6 (HHV6) were evaluated to determine overall result variability and then were stratified by assay manufacturer. The impact of calibration to international units/ml (CMV and EBV) on variability was also determined. Viral loads showed a high degree of interlaboratory variability for all tested viruses, with interquartile ranges as high as 1.46 log10 copies/ml and the overall range for a given sample up to 5.66 log(10) copies/ml. Some improvement in result variability was seen when international units were adopted. This was particularly the case for EBV viral load results. Variability in viral load results remains a challenge across all viruses tested here; introduction of international quantitative standards may help reduce variability and does so more or less markedly for certain viruses.

引用

页码：423 / 430

页数：8

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