Studies of hTERT DNA methylation assays on the human age prediction

被引：8

作者：

Xin, Ye ^{[1
]}

Dong, Kaikai ^{[1
]}

Cao, Fangqi ^{[1
,2
]}

Tian, Yuxiang ^{[3
]}

Sun, Jing ^{[4
]}

Peng, Min ^{[4
]}

Liu, Wenbin ^{[2
]}

Shi, Ping ^{[1
,4
]}

机构：

[1] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

[2] Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, Zhongshan North 1 Rd, Shanghai 200083, Peoples R China

[3] Shanghai Xuhui Dist Dahua Hosp, Dept Clin Lab, Shanghai 200237, Peoples R China

[4] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Qinghai Tibet Plateau Biol R, Xiguan Ave 59, Xining 810001 11, Qinghai, Peoples R China

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2019年 / 133卷 / 05期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; Age prediction; MSP; Real-time PCR; PROMOTER;

D O I：

10.1007/s00414-019-02076-3

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

As an important aspect of epigenetics, DNA methylation has been proven to be suitable for forensic DNA analysis. By detecting changes in DNA methylation, it is desirable to construct a model of age patterns associated with it to infer the age of the individual. The hTERT gene methylation is closely related to tumors, but there are few reports on the relationship between hTERT gene promoter methylation and age. In this study, we utilized the methylation-specific polymerase chain reaction and real-time PCR (relative quantification and absolute quantification) approach to explore the connection between hTERT DNA methylation and age prediction. We fit three models for age prediction based on methylation assay for 90 blood samples from donors aged 1-79 years old. Among them, the model of absolute quantification of real-time enabled the age prediction with R-2 = 0.9634. We verified the linear regression model with a validation set of 30 blood samples where prediction average error was 4.29 years. Generally, this reliable method improves the DNA methylation analysis of forensic samples.

引用

页码：1333 / 1339

页数：7

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