A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

被引：69

作者：

Francois, P ^{[1
]}

Renzi, G

Pittet, D

Bento, M

Lew, D

Harbarth, S

Vaudaux, P

Schrenzel, J

机构：

[1] Univ Hosp Geneva, Div Infect Dis, Genom Res Lab, CH-1211 Geneva 14, Switzerland

[2] Univ Hosp Geneva, Div Infect Dis, Clin Microbiol Lab, CH-1211 Geneva 14, Switzerland

[3] Univ Hosp Geneva, Infect Control Program, CH-1211 Geneva 14, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 07期

关键词：

D O I：

10.1128/JCM.42.7.3309-3312.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

引用

页码：3309 / 3312

页数：4

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