Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275

被引：22

作者：

Kosgey, Zennah C. ^{[1
,2
]}

Edae, Erena A. ^{[2
]}

Dill-Macky, Ruth ^{[2
]}

Jin, Yue ^{[2
,3
]}

Bulbula, Worku Denbel ^{[2
,4
]}

Gemechu, Ashenafi ^{[4
]}

Macharia, Godwin ^{[1
]}

Bhavani, Sridhar ^{[5
]}

Randhawa, Mandeep S. ^{[6
]}

Rouse, Matthew N. ^{[2
,3
]}

机构：

[1] Kenya Agr & Livestock Res Org, Njoro, Kenya

[2] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA

[3] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA

[4] Ethiopian Inst Agr Res, Debre Zeit Agr Res Ctr, Bishoftu, Ethiopia

[5] Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco, Mexico

[6] CIMMYT World Agroforestry Ctr ICRAF, Nairobi, Kenya

来源：

FRONTIERS IN PLANT SCIENCE | 2021年 / 11卷

关键词：

Triticum aestivum; Puccinia graminis f; sp; tritici; genetics; QTL; disease resistance; stem rust; Ug99; F-SP TRITICI; ADULT-PLANT RESISTANCE; UG99 RACE GROUP; POWDERY MILDEW; GENE; VIRULENCE; MARKERS; ALLELE; TTKS; IDENTIFICATION;

D O I：

10.3389/fpls.2020.609659

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.

引用

页数：13

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