Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity

被引：291

作者：

Janzen, Daniel H. ^{[1
]}

Hallwachs, Winnie ^{[1
]}

Blandin, Patrick ^{[2
]}

Burns, John M. ^{[3
]}

Cadiou, Jean-Marie

Chacon, Isidro ^{[4
]}

Dapkey, Tanya ^{[1
]}

Deans, Andrew R. ^{[5
]}

Epstein, Marc E. ^{[6
]}

Espinoza, Bernardo ^{[4
]}

Franclemont, John G.

Haber, William A. ^{[7
]}

Hajibabaei, Mehrdad ^{[8
]}

Hall, Jason P. W. ^{[3
]}

Hebert, Paul D. N. ^{[9
]}

Gauld, Ian D.

Harvey, Donald J. ^{[3
]}

Hausmann, Axel ^{[10
]}

Kitching, Ian J. ^{[11
]}

Lafontaine, Don ^{[12
]}

Landry, Jean-Francois ^{[12
]}

Lemaire, Claude

Miller, Jacqueline Y. ^{[13
]}

Miller, James S.

Miller, Lee

Miller, Scott E. ^{[3
]}

Montero, Jose ^{[4
]}

Munroe, Eugene

Green, Suzanne Rab ^{[14
]}

Ratnasingham, Sujeevan ^{[8
]}

Rawlins, John E. ^{[15
]}

Robbins, Robert K. ^{[3
]}

Rodriguez, Josephine J. ^{[16
]}

Rougerie, Rodolphe ^{[8
]}

Sharkey, Michael J. ^{[17
]}

Smith, M. Alex ^{[8
]}

Solis, M. Alma ^{[18
]}

Sullivan, J. Bolling

Thiaucourt, Paul

Wahl, David B. ^{[19
]}

Weller, Susan J. ^{[20
]}

Whitfield, James B. ^{[20
]}

Willmott, Keith R. ^{[13
]}

Wood, D. Monty ^{[12
]}

Woodley, Norman E. ^{[18
]}

Wilson, John J. ^{[8
]}

机构：

[1] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

[2] Museum Natl Hist Nat, F-75005 Paris, France

[3] Smithsonian Inst, Natl Museum Nat Hist, Dept Entomol, Washington, DC 20013 USA

[4] Inst Nacl Biodiversidad, Santo Domingo De Heredia, Costa Rica

[5] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA

[6] Calif Dept Food & Agr, Plant Pest Diagnost Branch, Sacramento, CA 95832 USA

[7] Missouri Bot Garden, St Louis, MO 63166 USA

[8] Univ Guelph, Canadian Ctr DNA Barcoding, Biodivers Inst Ontario, Guelph, ON N1G 2W1, Canada

[9] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada

[10] Zool State Collect Munich, Sekt Lepidoptera, D-81247 Munich, Germany

[11] Nat Hist Museum, Dept Entomol, London SW7 5BD, England

[12] Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, Canada

[13] Univ Florida, McGuire Ctr Lepidoptera & Biodivers, Florida Museum Nat Hist, Gainesville, FL 32611 USA

[14] Amer Museum Nat Hist, Div Invertebrate Zool, New York, NY 10024 USA

[15] Carnegie Museum Nat Hist, Sect Invertebrate Zool, Pittsburgh, PA 15213 USA

[16] Univ Illinois, Urbana, IL 61801 USA

[17] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA

[18] Smithsonian Inst, SEL, USDA, Washington, DC 20013 USA

[19] Amer Entomol Inst, Gainesville, FL 32608 USA

[20] Univ Minnesota, Bell Museum Nat Hist, Minneapolis, MN 55455 USA

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷

基金：

美国国家科学基金会; 加拿大自然科学与工程研究理事会;

关键词：

Area de Conservacion Guanacaste; Braconidae; COI; Costa Rica; DNA barcoding; Lepidoptera; species identification; Tachinidae; taxonomy; tropical biodiversity inventory; PARASITOID FLIES DIPTERA; SKIPPER BUTTERFLIES; GROUP HYMENOPTERA; LEPIDOPTERA; WASPS; GENUS; IDENTIFICATION; DIVERGENCE; DIVERSITY; TAXONOMY;

D O I：

10.1111/j.1755-0998.2009.02628.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding - the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species - was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to 'variation' or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.

引用

页码：1 / 26

页数：26

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