Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells

被引：49
作者：
Cai, Jiaqin [1 ]
Sun, Hong [1 ]
Zheng, Bin [2 ]
Xie, Mumu [1 ]
Xu, Chenxia [2 ]
Zhang, Guifeng [1 ]
Huang, Xuhui [1 ]
Zhuang, Jie [1 ]
机构：
[1] Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Pharm, 134 Dong St, Fuzhou 350001, Fujian, Peoples R China
[2] Fujian Med Univ, Dept Pharm, Union Hosp, Fuzhou 350001, Fujian, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2021年 / 23卷 / 01期
关键词：
tamoxifen-resistance; epithelial-mesenchymal transition; breast cancer; H19 imprinted maternally expressed transcript; curcumin; EXPRESSION; THERAPY; SNAIL;
D O I：
10.3892/mmr.2020.11651
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The H19 long non-coding RNA is involved in the development of tamoxifen resistance in breast cancer. However, the relationship between H19 and the metastatic potential and treatment options for tamoxifen-resistant (TAMR) breast cancer is not completely understood. Curcumin inhibits cellular proliferation, migration and invasiveness in several cancer types, including pancreatic cancer, breast cancer and chronic myeloid leukemia. The present study aimed to investigate the role of H19 in MCF-7/TAMR cell epithelial-mesenchymal transition (EMT), migration and invasiveness, and to assess the ability of curcumin to inhibit H19-mediated effects. Reverse transcription-quantitative PCR and western blot analysis were conducted to detect the gene or protein expression. Cell Counting Kit-8, wound healing and Transwell invasion assays were performed to estimate the capabilities of cell viability, invasion and migration. H19 overexpression enhanced MCF-7/TAMR cell EMT, invasion and migration by upregulating Snail. Furthermore, curcumin notably decreased the expression levels of epithelial marker E-cadherin and markedly increased the expression levels of mesenchymal marker N-cadherin in MCF-7/TAMR cells compared with the control group. In addition, following treatment with curcumin for 48 h, H19 expression was decreased in a dose-dependent manner. Moreover, curcumin treatment for 48 h significantly attenuated H19-induced alterations in N-cadherin and E-cadherin expression levels. Curcumin also prevented H19-induced invasion and migration. The present study indicated that H19 may serve as a promoting factor of EMT, invasion and migration in MCF-7/TAMR cells, suggesting that curcumin may prevent H19-associated metastasis. Therefore, curcumin may serve as a promising therapeutic drug for patients with TAMR breast cancer.
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页数：9
相关论文
共 32 条
[1]  
Al Khatib A M, 2019, Curr Health Sci J, V45, P204, DOI 10.12865/CHSJ.45.02.12
[2]   Long Non-Coding RNA H19 Acts as an Estrogen Receptor Modulator that is Required for Endocrine Therapy Resistance in ER+ Breast Cancer Cells [J].
Basak, Pratima ;
Chatterjee, Sumanta ;
Bhat, Vasudeva ;
Su, Alice ;
Jin, Hyerang ;
Lee-Wing, Victoria ;
Liu, Qian ;
Hu, Pingzhao ;
Murphy, Leigh C. ;
Raouf, Afshin .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 51 (04) :1518-1532
[3]   Adjuvant Endocrine Therapy for Women With Hormone Receptor-Positive Breast Cancer: American Society of Clinical Oncology Clinical Practice Guideline Focused Update [J].
Burstein, Harold J. ;
Temin, Sarah ;
Anderson, Holly ;
Buchholz, Thomas A. ;
Davidson, Nancy E. ;
Gelmon, Karen E. ;
Giordano, Sharon H. ;
Hudis, Clifford A. ;
Rowden, Diana ;
Solky, Alexander J. ;
Stearns, Vered ;
Winer, Eric P. ;
Griggs, Jennifer J. .
JOURNAL OF CLINICAL ONCOLOGY, 2014, 32 (21) :2255-+
[4]   Curcumin inhibits hypoxia-induced epithelial-mesenchymal transition in pancreatic cancer cells via suppression of the hedgehog signaling pathway [J].
Cao, Lei ;
Xiao, Xue ;
Lei, Jianjun ;
Duan, Wanxing ;
Ma, Qingyong ;
Li, Wei .
ONCOLOGY REPORTS, 2016, 35 (06) :3728-3734
[5]   Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells [J].
Cao, Meng-Ting ;
Liu, Hui-Fang ;
Liu, Zhi-Gang ;
Xiao, Ping ;
Chen, Jing-Jing ;
Tan, Yuan ;
Jiang, Xiao-Xin ;
Jiang, Zhi-Chao ;
Qiu, Yu ;
Huang, Hong-Jun ;
Zhang, Qiu-Gui ;
Jiang, Guan-Min .
ONCOTARGET, 2017, 8 (65) :108498-108508
[6]   Tamoxifen Resistance in Breast Cancer [J].
Chang, Minsun .
BIOMOLECULES & THERAPEUTICS, 2012, 20 (03) :256-267
[7]   Relevance of breast cancer hormone receptors and other factors to the efficacy of adjuvant tamoxifen: patient-level meta-analysis of randomised trials [J].
Davies, C. ;
Godwin, J. ;
Gray, R. ;
Clarke, M. ;
Darby, S. ;
McGale, P. ;
Wang, Y. C. ;
Peto, R. ;
Pan, H. C. ;
Cutter, D. ;
Taylor, C. ;
Ingle, J. .
LANCET, 2011, 378 (9793) :771-784
[8]   4-protein signature predicting tamoxifen treatment outcome in recurrent breast cancer [J].
De Marchi, Tommaso ;
Liu, Ning Qing ;
Stingl, Cristoph ;
Timmermans, Mieke A. ;
Smid, Marcel ;
Look, Maxime P. ;
Tjoa, Mila ;
Braakman, Rene B. H. ;
Opdam, Mark ;
Linn, Sabine C. ;
Sweep, Fred C. G. J. ;
Span, Paul N. ;
Kliffen, Mike ;
Luider, Theo M. ;
Foekens, John A. ;
Martens, John W. M. ;
Umar, Arzu .
MOLECULAR ONCOLOGY, 2016, 10 (01) :24-39
[9]   Curcumin inhibits invasive capabilities through epithelial mesenchymal transition in breast cancer cell lines [J].
Gallardo, Marcela ;
Calaf, Gloria M. .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 49 (03) :1019-1027
[10]   Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells [J].
Hu, Shan ;
Xu, Yingchun ;
Meng, Liwei ;
Huang, Liming ;
Sun, He .
EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 16 (02) :1266-1272
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