Expression of a Pteris vittata glutaredoxin PvGRX5 in transgenic Arabidopsis thaliana increases plant arsenic tolerance and decreases arsenic accumulation in the leaves

被引：48

作者：

Sundaram, Sabarinath ^{[1
]}

Wu, Shan ^{[1
]}

Ma, Lena Q. ^{[2
]}

Rathinasabapathi, Bala ^{[1
]}

机构：

[1] Univ Florida, Dept Hort Sci, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Soil & Water Sci, Gainesville, FL 32611 USA

来源：

PLANT CELL AND ENVIRONMENT | 2009年 / 32卷 / 07期

关键词：

Arabidopsis thaliana; Pteris vittata; arsenate reduction; arsenic accumulation; arsenic tolerance; arsenic transport; glutaredoxin; heavy metal; RICE ORYZA-SATIVA; SACCHAROMYCES-CEREVISIAE; PHYTOCHELATIN SYNTHASE; CONTAMINATED SOILS; REDUCTASE; FERN; HYPERACCUMULATION; WATER; PHYTOREMEDIATION; SYSTEMS;

D O I：

10.1111/j.1365-3040.2009.01963.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Chinese brake fern Pteris vittata hyperaccumulates arsenic in its fronds. In a study to identify brake fern cDNAs in arsenic resistance, we implicated a glutaredoxin, PvGRX5, because when expressed in Escherichia coli, it improved arsenic tolerance in recombinant bacteria. Here, we asked whether PvGRX5 transgenic expression would alter plant arsenic tolerance and metabolism. Two lines of Arabidopsis thaliana constitutively expressing PvGrx5 cDNA were compared with vector control and wild-type lines. PvGRX5-expressors were significantly more tolerant to arsenic compared with control lines based on germination, root growth and whole plant growth under imposed arsenic stress. PvGRX5-expressors contained significantly lower total arsenic compared with control lines following treatment with arsenate. Additionally, PvGRX5-expressors were significantly more efficient in their arsenate reduction in vivo. Together, our results indicate that PvGRX5 has a role in arsenic tolerance via improving arsenate reduction and regulating cellular arsenic levels. Paradoxically, our results suggest that PvGRX5 from the arsenic hyperaccumulator fern can be used in a novel biotechnological solution to decrease arsenic in crops.

引用

页码：851 / 858

页数：8

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