Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols)

被引：28
作者：
Wang, Shuang [1 ]
Redmile-Gordon, Marc [2 ]
Mortimer, Monika [3 ,4 ]
Cai, Peng [1 ]
Wu, Yichao [1 ]
Peacock, Caroline L. [5 ]
Gao, Chunhui [1 ]
Huang, Qiaoyun [1 ]
机构：
[1] Huazhong Agr Univ, Coll Resources & Environm, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
[2] Royal Hort Soc, Dept Environm Hort, Wisley GU23 6QB, Surrey, England
[3] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Earth Res Inst, Santa Barbara, CA 93106 USA
[4] Univ Calif Santa Barbara, UC CEIN, Santa Barbara, CA 93106 USA
[5] Univ Leeds, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2019年 / 135卷
基金：
中国国家自然科学基金;
关键词：
Soil biofilms; Extracellular polymeric substances; Polysaccharide; Cation exchange resin; Easily extractable glomalin; ACTIVATED-SLUDGE; ORGANIC-MATTER; EFFICIENCY; BIOFILM;
D O I：
10.1016/j.soilbio.2019.05.014
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Extracellular polymeric substances (EPS) have many beneficial functions in soils. Accurate quantification of EPS in soils is crucial. Here, five methods were compared for their suitability for extraction of EPS from Ultisols: hot water extractable polysaccharide (HWEP), hot dilute acid extractable polysaccharide (HDAEP), easily extractable glomalin (EEG), sodium sulfide (SS) and cation exchange resin (CER) method. Humic-acid equivalent (HAE) was used as an indicator for extracellular contamination and ATP for quantifying intracellular contamination from cell lysis. Among the tested methods, CER resulted in EPS extraction with minimal contamination. Therefore, we propose that CER is currently the most appropriate method for extraction of EPS from Ultisols.
引用
收藏
页码：283 / 285
页数：3
相关论文
共 50 条
  • [31] Stratification of Extracellular Polymeric Substances (EPS) for Aggregated Anammox Microorganisms
    Jia, Fangxu
    Yang, Qing
    Liu, Xiuhong
    Li, Xiyao
    Li, Baikun
    Zhang, Liang
    Peng, Yongzhen
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2017, 51 (06) : 3260 - 3268
  • [32] Microbial Extracellular Polymeric Substances (EPS) in Fresh Water Sediments
    Gerbersdorf, Sabine Ulrike
    Westrich, Bernhard
    Paterson, David M.
    MICROBIAL ECOLOGY, 2009, 58 (02) : 334 - 349
  • [33] Extraction of extracellular polymeric substances in activated sludge using sequential extraction
    Sun, LianPeng
    Chen, Lili
    Guo, Wu Zhen
    Ye, Tingjin
    Yang, Ying
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2015, 90 (08) : 1448 - 1454
  • [34] Composition of Extracellular Polymeric Substances (EPS) produced by Flavobacterium columnare isolated from tropical fish in Brazil
    Sebastiao, Fernanda de Alexandre
    Pilarski, Fabiana
    Franco Lemos, Manoel Victor
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2013, 44 (03) : 861 - 864
  • [35] Degradation of extracellular polymeric substances (EPS) extracted from activated sludge by low-concentration ozonation
    Meng, Lu
    Xi, Jinying
    Yeung, Marvin
    CHEMOSPHERE, 2016, 147 : 248 - 255
  • [36] Characterising soil extracellular polymeric substances (EPS) by application of spectral-chemometrics and deconstruction of the extraction process
    Zhang, Ming
    Xu, Ying
    Xiao, Ke-Qing
    Gao, Chun-Hui
    Wang, Shuang
    Zhu, Di
    Wu, Yichao
    Huang, Qiaoyun
    Cai, Peng
    CHEMICAL GEOLOGY, 2023, 618
  • [37] The important implications of particulate substrate in determining the physicochemical characteristics of extracellular polymeric substances (EPS) in activated sludge
    Wang, Bin-Bin
    Peng, Dang-Cong
    Hou, Yin-Ping
    Li, Hui-Juan
    Pei, Li-Ying
    Yu, Li-Fang
    WATER RESEARCH, 2014, 58 : 1 - 8
  • [38] Interaction of acetamiprid with extracellular polymeric substances (EPS) from activated sludge: A fluorescence study
    Song, Wenjuan
    Mu, Guijin
    Zhang, Daoyong
    Pan, Xiangliang
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (45): : 7667 - 7673
  • [39] Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge
    Liang, Zhiwei
    Li, Wenhong
    Yang, Shangyuan
    Du, Ping
    CHEMOSPHERE, 2010, 81 (05) : 626 - 632
  • [40] Effects of enzymatic treatment on the reduction of extracellular polymeric substances (EPS) from biofouled membranes
    Kim, Lan Hee
    Kim, Sung-Jo
    Kim, Chang-Min
    Shin, Myung Seop
    Kook, Seungho
    Kim, In S.
    DESALINATION AND WATER TREATMENT, 2013, 51 (31-33) : 6355 - 6361
12345