Nitrogen removal by heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas sp. DM02: Removal performance, mechanism and immobilized application for real aquaculture wastewater treatment

被引：112
作者：
Deng, Min [1 ]
Zhao, Xiaoli [2 ]
Senbati, Yeerken [1 ,3 ]
Song, Kang [1 ,3 ]
He, Xugang [4 ]
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[2] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[4] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2021年 / 322卷
基金：
中国国家自然科学基金;
关键词：
Nitrogen removal; Pseudomonas; Heterotrophic nitrification-aerobic denitrification; napA; Immobilized carrier; AMMONIUM REMOVAL; NITRIFICATION; DENITRIFICATION; ASSIMILATION;
D O I：
10.1016/j.biortech.2020.124555
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A bacterial strain was isolated and identified as Pseudomonas sp. DM02 from an aquaculture system. Strain DM02 showed efficient heterotrophic nitrification-aerobic denitrification capability. Total ammonia nitrogen (TAN, 10 mg/L) could be completely removed by strain DM02 within 12 h under low nutrient condition. Nitrogen mass balance indicated that 70.8% of the initial TAN was translated into gaseous nitrogen and 28.1% was converted into intracellular nitrogen. Various carbon sources can be used for nitrate removal (>95% within 28 h). The optimal conditions for TAN, nitrate and nitrite removal were pH 7 with carbon/nitrogen (C/N) ratios of 8, 12 and 12, respectively. The napA, nirK, and nosZ functional genes were successful amplified from strain DM02. Both bioaugmentation and immobilized technology of strain DM02 present ability (>88%) for continuous treatment of real aquaculture wastewater. This research indicated a great potential for practical application of Pseudomonas sp. DM02 in aquaculture wastewater treatment.
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页数：10
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