Confocal laser scanning fluorescence microscopy with a visible continuum source

被引：64

作者：

McConnell, G ^{[1
]}

机构：

[1] Univ Strathclyde, Strathclyde Inst Biomed Sci, Ctr Biophoton, Glasgow G4 0NR, Lanark, Scotland

来源：

OPTICS EXPRESS | 2004年 / 12卷 / 13期

关键词：

D O I：

10.1364/OPEX.12.002844

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue. (C) 2004 Optical Society of America.

引用

页码：2844 / 2850

页数：7

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