Small but Powerful: Co-Assembly of Polyether-Based Triblock Terpolymers into Sub-30 nm Micelles and Synergistic Effects on Cellular Interactions

被引：24

作者：

Barthel, Markus J. ^{[1
,2
,3
]}

Rinkenauer, Alexandra C. ^{[1
,2
]}

Wagner, Michael ^{[1
,2
]}

Mansfeld, Ulrich ^{[1
,2
]}

Hoeppener, Stephanie ^{[1
,2
]}

Czaplewska, Justyna A. ^{[1
,2
]}

Gottschaldt, Michael ^{[1
,2
]}

Traeger, Anja ^{[1
,2
]}

Schacher, Felix H. ^{[1
,2
]}

Schubert, Ulrich S. ^{[1
,2
,3
]}

机构：

[1] Univ Jena, Lab Organ & Macromol Chem IOMC, D-07743 Jena, Germany

[2] Univ Jena, Jena Ctr Soft Matter JCSM, D-07743 Jena, Germany

[3] Dutch Polymer Inst DPI, NL-5600 AX Eindhoven, Netherlands

来源：

BIOMACROMOLECULES | 2014年 / 15卷 / 07期

关键词：

RESPONSIVE MULTICOMPARTMENT MICELLES; TRANSMISSION ELECTRON-MICROSCOPY; MULTIANGLE LIGHT-SCATTERING; BLOCK-COPOLYMER MICELLES; FIELD-FLOW FRACTIONATION; CONTROLLED DRUG-RELEASE; GENE DELIVERY; TRANSFECTION EFFICIENCY; POLY(ETHYLENE OXIDE); POLYMERIC MICELLES;

D O I：

10.1021/bm5002894

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a versatile ABC triblock terpolymer platform based on poly(ethylene oxide)-block-poly(allyl glycidyl ether)-block-poly(tert-butyl glycidyl ether) (PEO-b-PAGE-b-PtBGE) and subsequent functionalization of the PAGE segment with thiogalactose (hydroxyl), cysteamine (amino), and 2-mercaptopropionic acid (carboxy) by thiolene chemistry. These materials are used to prepare core-shell-corona micelles with a PtBGE core, a PAGE shell, and a PEO corona and sizes below 30 nm in aqueous media. We investigate the influence of different functional groups on micelle formation and cellular uptake. Moreover, co-assembly of differently functionalized materials allows to create micelles with a mixed shell and adjustable charge and, in that way, important characteristics such as cell uptake or cytotoxicity can be controlled. Furthermore, we demonstrate that even the uptake mechanism depends on the substitution pattern of the underlying triblock terpolymer.

引用

页码：2426 / 2439

页数：14

共 71 条

[1]

Albanese A, 2012, ANNU REV BIOMED ENG, V14, P1, DOI [10.1146/annurev-bioeng-071811-150124, 10.1146/annurev.bioeng-071811-150124]

[2] Factors affecting the clearance and biodistribution of polymeric nanoparticles [J].