Effective method for purification of lysozyme from human urine

被引：6

作者：

Takai, I ^{[1
]}

Oda, O ^{[1
]}

Shinzato, T ^{[1
]}

Ohbayashi, K ^{[1
]}

Yamanaka, N ^{[1
]}

Maeda, K ^{[1
]}

机构：

[1] NAGOYA MEM HOSP,BIODYNAM RES INST,NAGOYA,AICHI,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 685卷 / 01期

关键词：

lysozyme; proteins; enzymes;

D O I：

10.1016/0378-4347(96)00161-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysozyme in the urine of a hemodialysis patient was purified in two steps: DEAE Sephadex chromatography followed by Sephacryl chromatography. The Sephacryl S-100 column chromatographed fraction showing lytic activity was proven to give one band on SDS-PAGE and to have a molecular mass of 14 500, in agreement with that of lysozyme. The N-terminal amino acid sequence of this purified protein was identical to that of lysozyme. These results indicate that the protein purified was indeed lysozyme. The specific affinity of lysozyme for Sephacryl S-100 may explain the greater purity of the same protein isolated by this method.

引用

页码：21 / 25

页数：5

共 16 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] PURIFICATION AND CHARACTERIZATION OF A LYSOZYME FROM GOOSE EGG WHITE [J].