Production, partial purification and characterization of organic solvent tolerant lipase from Burkholderia multivorans V2 and its application for ester synthesis

被引：110

作者：

Dandavate, Vrushah ^{[1
]}

Jinjala, Jayesh ^{[1
]}

Keharia, Haresh ^{[1
]}

Madamwar, Datta ^{[1
]}

机构：

[1] Sardar Patel Univ, BRD Sch Biosci, Vallabh Vidyanagar 388120, Gujarat, India

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 13期

关键词：

Burkholeria multivorans lipase; Organic solvent tolerance; Partial purification; Ester synthesis; CHEMICAL-MODIFICATION; ALKALINE LIPASE; PSEUDOMONAS SP; ENZYME; COMPLEXES; S5;

D O I：

10.1016/j.biortech.2009.02.011

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Burkholderia multivorans V2 (BMV2) isolated from soil was found to produce an extracellular solvent tolerant lipase (6.477 U/mL). This lipase exhibited maximum stability in n-hexane retaining about 97.8% activity for 24 h. After performing statistical optimization of medium components for lipase production, a 2.2-fold (14 U/mL) enhancement in the lipase production was observed. The crude lipase from BMV2 was partially purified by ultrafiltration and gel permeation chromatography with 24.64-fold purification. The K-m and V-max values for partially purified BMV2 lipase were found to be 1.56 mM and 5.62 mu moles/mg min. The metal ions Ca2+, Mg2+ and Mn2+ had stimulatory effect on lipase activity, whereas Cu2+, Fe2+ and Zn2+ strongly inhibited the lipase activity. EDTA and PMSF at 10 mM concentration strongly inhibited the lipase activity. Non-ionic and anionic surfactants stimulated the lipase activity. BMV2 lipase was proved to be efficient in synthesis of ethyl butyrate ester under non-aqueous environment. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：3374 / 3381

页数：8

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