In vitro biocompatibility assessment of poly(ε-caprolactone) films using L929 mouse fibroblasts

被引：236
作者：
Serrano, MC
Pagani, R
Vallet-Regí, M
Peña, J
Rámila, A
Izquierdo, I
Portolés, MT
机构：
[1] Univ Complutense, Fac Chem, Dept Biochem & Mol Biol 1, E-28040 Madrid, Spain
[2] Univ Complutense, Fac Chem, Dept Inorgan & Bioinorgan Chem, E-28040 Madrid, Spain
来源：
BIOMATERIALS | 2004年 / 25卷 / 25期
关键词：
polycaprolactone; biocompatibility; cell adhesion; cell proliferation; MTT assays; fibroblast;
D O I：
10.1016/j.biomaterials.2004.01.037
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Biodegradable and biocompatible materials are the basis for tissue engineering. As an initial step for developing vascular grafts, the in vitro biocompatibility of poly(epsilon-caprolactone) (PCL), recently Suggested for several clinical applications, was evaluated ill this study using L929 mouse fibroblasts. Different cellular aspects were analyzed in order to know the cell viability during cell culture oil PCL films: adhesion, proliferation, morphology, LDH release and mitochondrial function. Since topography and other Surface characteristics of materials play ail essential part in cell adhesion, PCL membranes with either smooth or rough surface were prepared, characterized and used to carry out cell cultures. During short culture times, PCL produced a significant stimulation of mitochondrial activity evaluated by reduction of the MTT reagent. The results provide evidences of good adhesion, growth, viability, morphology and mitochondrial activity of cells on PCL films. Therefore, it can be Concluded that PCL is a Suitable Land biocompatible material as a scaffold for vascular graft development. (C) 2004 Elsevier Ltd. All rights reserved.
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收藏
页码：5603 / 5611
页数：9
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