Characterization and promoter analysis of the mouse nestin gene

被引：31
作者：
Cheng, LP [1 ]
Jin, ZG [1 ]
Liu, L [1 ]
Yan, Y [1 ]
Li, TT [1 ]
Zhu, XL [1 ]
Jing, NH [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Lab Stem Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
来源：
FEBS LETTERS | 2004年 / 565卷 / 1-3期
基金：
中国国家自然科学基金;
关键词：
mouse nestin gene; promoter; Sp1; sp3; p19; cell;
D O I：
10.1016/j.febslet.2004.03.097
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The intermediate filament protein nestin is expressed in the neural stem cells of the developing central nervous system (CNS). Promoter analysis revealed that the minimal promoter of the mouse nestin gene resides in the region -11 to + 183 of the 5' non-coding and upstream flanking region, and that two adjacent Sp1-binding sites are necessary for promoter activity. Electrophoretic mobility-shift assays (EMSA) and supershift assays showed that Sp1 and Sp3 proteins selectively bind to the upstream Sp1 site. These results demonstrate an important functionality of Sp1 and Sp3 in regulating the expression of the mouse nestin gene. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：195 / 202
页数：8
相关论文
共 35 条
  • [1] Nestin expression in embryonic and adult human teeth under normal and pathological conditions
    About, I
    Laurent-Maquin, D
    Lendahl, U
    Mitsiadis, TA
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2000, 157 (01) : 287 - 295
  • [2] A RAPID MICROPREPARATION TECHNIQUE FOR EXTRACTION OF DNA-BINDING PROTEINS FROM LIMITING NUMBERS OF MAMMALIAN-CELLS
    ANDREWS, NC
    FALLER, DV
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (09) : 2499 - 2499
  • [3] FROM EMBRYONAL CARCINOMA-CELLS TO NEURONS - THE P19 PATHWAY
    BAIN, G
    RAY, WJ
    YAO, M
    GOTTLIEB, DI
    [J]. BIOESSAYS, 1994, 16 (05) : 343 - 348
  • [4] Structural and functional characterization of the human and mouse fibulin-1 gene promoters: role of Sp1 and Sp3
    Castoldi, M
    Chu, ML
    [J]. BIOCHEMICAL JOURNAL, 2002, 362 : 41 - 50
  • [5] Cheng LP, 2000, ACTA BIOCH BIOPH SIN, V32, P649
  • [6] NESTIN MESSENGER-RNA EXPRESSION CORRELATES WITH THE CENTRAL-NERVOUS-SYSTEM PROGENITOR-CELL STATE IN MANY, BUT NOT ALL, REGIONS OF DEVELOPING CENTRAL-NERVOUS-SYSTEM
    DAHLSTRAND, J
    LARDELLI, M
    LENDAHL, U
    [J]. DEVELOPMENTAL BRAIN RESEARCH, 1995, 84 (01): : 109 - 129
  • [7] DAHLSTRAND J, 1992, J CELL SCI, V103, P589
  • [8] TRANSCRIPTIONAL ACTIVATION OF THE NEURONAL PERIPHERIN-ENCODING GENE DEPENDS ON A G+C-RICH ELEMENT THAT BINDS SP1 IN-VITRO AND IN-VIVO
    FERRARI, N
    DESMARAIS, D
    ROYAL, A
    [J]. GENE, 1995, 159 (02) : 159 - 165
  • [9] FREDERIKSEN K, 1988, J NEUROSCI, V8, P1144
  • [10] The intermediate filament protein nestin occurs transiently in differentiating testis of rat and mouse
    Frojdman, K
    Pelliniemi, LJ
    Lendahl, U
    Virtanen, I
    Eriksson, JE
    [J]. DIFFERENTIATION, 1997, 61 (04) : 243 - 249
1234