Biotechnological potential of endophytic actinomycetes associated with Asteraceae plants: isolation, biodiversity and bioactivities

被引:15
作者
Tanvir, Rabia [1 ]
Sajid, Imran [1 ]
Hasnain, Shahida [1 ,2 ]
机构
[1] Univ Punjab, Dept Microbiol & Mol Genet, Lahore 54590, Punjab, Pakistan
[2] Women Univ, Dept Microbiol & Mol Genet, Multan, Punjab, Pakistan
关键词
Actinomycetes; Anti-microbial activities; Anti-oxidant activities; Asteraceae; Bioactivities; Biodiversity; Cytotoxic activity; Endophytes; L;
D O I
10.1007/s10529-013-1430-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Endophytic actinomycetes from five Asteraceae plants were isolated and evaluated for their bioactivities. From Parthenium hysterophorus, Ageratum conyzoides, Sonchus oleraceus, Sonchus asper and Hieracium canadense, 42, 45, 90, 3, and 2 isolates, respectively, were obtained. Of the isolates, 86 (47.2 %) showed antimicrobial activity. Majority of the isolates were recovered from the roots (n = 127, 69.7 %). The dominant genus was Streptomyces (n = 96, 52.7 %), while Amycolatopsis, Pseudonocardia, Nocardia and Micromonospora were also recovered. Overall, 36 of the 86 isolates were significantly bioactivity while 18 (20.9 %) showed strong bioactivity. In total, 52.1 and 66.6 % showed potent cytotoxicity and antioxidant activities. The LC50 for 15 strains was < 20 mu g/ml. Compared to the ascorbate standard (EC50 0.34 mu g/ml), all isolates gave impressive results with notable EC50 values of 0.65, 0.67, 0.74 and 0.79 mu g/ml.
引用
收藏
页码:767 / 773
页数:7
相关论文
共 21 条
[1]   Anticancer and antiradical scavenging activity of Ageratum conyzoides L. (Asteraceae) [J].
Adebayo, A. H. ;
Tan, N. H. ;
Akindahunsi, A. A. ;
Zeng, G. Z. ;
Zhang, Y. M. .
PHARMACOGNOSY MAGAZINE, 2010, 6 (21) :62-66
[2]   Determination of minimum inhibitory concentrations [J].
Andrews, JM .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 :5-16
[3]   Multiple, Novel Biologically Active Endophytic Actinomycetes Isolated from Upper Amazonian Rainforests [J].
Bascom-Slack, Carol A. ;
Ma, Cong ;
Moore, Emily ;
Babbs, Beatrice ;
Fenn, Kathleen ;
Greene, Joshua S. ;
Hann, Bradley D. ;
Keehner, Jocelyn ;
Kelley-Swift, Elizabeth G. ;
Kembaiyan, Vivek ;
Lee, Sun Jin ;
Li, Puyao ;
Light, David Y. ;
Lin, Emily H. ;
Schorn, Michelle A. ;
Vekhter, Daniel ;
Boulanger, Lori-Ann ;
Hess, W. M. ;
Vargas, Percy Nunez ;
Strobel, Gary A. ;
Strobel, Scott A. .
MICROBIAL ECOLOGY, 2009, 58 (02) :374-383
[4]   Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp (MSU-2110) endophytic on Monstera sp. [J].
Ezra, D ;
Castillo, UF ;
Strobel, GA ;
Hess, WM ;
Porter, H ;
Jensen, JB ;
Condron, MAM ;
Teplow, DB ;
Sears, J ;
Maranta, M ;
Hunter, M ;
Weber, B ;
Yaver, D .
MICROBIOLOGY-SGM, 2004, 150 :785-793
[5]  
GERAN RI, 1972, CANC CHEMOTHER REP, V3, P1
[6]   Biosynthetic Potential of Phylogenetically Unique Endophytic Actinomycetes from Tropical Plants [J].
Janso, Jeffrey E. ;
Carter, Guy T. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (13) :4377-4386
[7]   Insecticidal activity of Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst [J].
Jaya ;
Singh, Priyanka ;
Prakash, Bhanu ;
Dubey, N. K. .
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2014, 51 (09) :2210-2215
[8]   Remedies Containing Asteraceae Extracts A Prospective Observational Study of Prescribing Patterns and Adverse Drug Reactions in German Primary Care [J].
Jeschke, Elke ;
Ostermann, Thomas ;
Lueke, Claudia ;
Tabali, Manuela ;
Kroez, Matthias ;
Bockelbrink, Angelina ;
Witt, Claudia M. ;
Willich, Stefan N. ;
Matthes, Harald .
DRUG SAFETY, 2009, 32 (08) :691-706
[9]  
Kekuda T. R. P., 2010, Journal of Pharmacy Research, V3, P26
[10]   PREPARATION OF ARTIFICIAL SEAWATER [J].
KESTER, DR ;
DUEDALL, IW ;
CONNORS, DN ;
PYTKOWICZ, RM .
LIMNOLOGY AND OCEANOGRAPHY, 1967, 12 (01) :176-+