Electrophysiological variability in the SH-SY5Y cellular line

被引：32

作者：

Santillo, Silvia ^{[1
]}

Moriello, Aniello Schiano ^{[2
]}

Di Maio, Vito ^{[1
]}

机构：

[1] CNR, Ist Cibernet E Caianiello, I-80078 Pozzuoli, NA, Italy

[2] CNR, Inst Biomol Chem, I-80078 Pozzuoli, NA, Italy

来源：

GENERAL PHYSIOLOGY AND BIOPHYSICS | 2014年 / 33卷 / 01期

关键词：

Whole-cell patch-clamp; SH-SY5Y; Ion channels; NEUROTROPHIC FACTOR; RETINOIC ACID; CELLS; MODEL;

D O I：

10.4149/gpb_2013071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The basic electrophysiological properties of SH-SY5Y tumour cells have been studied by whole cell patch-clamp in voltage clamp configuration. The results shown the existence of a large variability of the response among individual cells in the same experimental conditions. Experiments conducted by using different ionic concentrations of the recording pipette filling solution, yielded a significant variability of peak current amplitude for all the filling solutions used. In addition, variability among groups was detected. The inter-group variability was not dependent on the different ionic components among the groups. Our results confirm earlier findings that this cell line is not constituted of an uniform population of cells. Moreover, from the present results we can conclude that these cells have diverse regulatory patterns of membrane conductance, probably due to both the expression as well as a non precise regulation of the membrane density of the different channels.

引用

页码：121 / 129

页数：9