Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells

被引：77

作者：

Gaedicke, Simone ^{[1
]}

Braun, Friederike ^{[2
,4
]}

Prasad, Shruthi ^{[1
,4
]}

Machein, Marcia ^{[3
]}

Firat, Elke ^{[1
]}

Hettich, Michael ^{[1
,4
]}

Gudihal, Ravindra ^{[5
]}

Zhu, Xuekai ^{[1
]}

Klingner, Kerstin ^{[6
]}

Schueler, Julia ^{[6
]}

Herold-Mende, Christel C. ^{[7
]}

Grosu, Anca-Ligia ^{[1
,8
]}

Behe, Martin ^{[2
,9
]}

Weber, Wolfgang ^{[2
,8
,10
]}

Maecke, Helmut ^{[2
,8
]}

Niedermann, Gabriele ^{[1
,8
]}

机构：

[1] Univ Hosp Freiburg, Dept Radiat Oncol, D-79106 Freiburg, Germany

[2] Univ Hosp Freiburg, Dept Nucl Med, D-79106 Freiburg, Germany

[3] Univ Hosp Freiburg, Dept Neurosurg, D-79106 Freiburg, Germany

[4] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany

[5] Agilent Technol India Pvt Ltd, Bangalore 560048, Karnataka, India

[6] Oncotest, D-79108 Freiburg, Germany

[7] Univ Heidelberg Hosp, Dept Neurosurg, D-69120 Heidelberg, Germany

[8] German Consortium Translat Canc Res, D-69120 Heidelberg, Germany

[9] Paul Scherrer Inst, Univ Zurich Hosp, Swiss Fed Inst Technol, Ctr Radiopharmaceut Sci, CH-5232 Villigen, Switzerland

[10] Mem Sloan Kettering Canc Ctr, Mol Imaging & Therapy Serv, New York, NY 10065 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 06期

关键词：

cancer stem cells; CSCs; glioblastoma; CLINICAL-IMPLICATIONS; HEMATOPOIETIC STEM; CANCER; GLIOBLASTOMA; GROWTH; AC133; POPULATION; EXPRESSION; THERAPY; MARKER;

D O I：

10.1073/pnas.1314189111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra-and extracranial tumor entities. Here we demonstrate the successful noninvasive detection of AC133(+) tumor stem cells by PET and near-infrared fluorescence molecular tomography in subcutaneous and orthotopic glioma xenografts using antibody-based tracers. Particularly, microPET with Cu-64-NOTA-AC133 mAb yielded high-quality images with outstanding tumor-to-background contrast, clearly delineating subcutaneous tumor stem cell-derived xenografts from surrounding tissues. Intracerebral tumors as small as 2-3 mm also were clearly discernible, and the microPET images reflected the invasive growth pattern of orthotopic cancer stem cell-derived tumors with low density of AC133(+) cells. These data provide a basis for further preclinical and clinical use of the developed tracers for high-sensitivity and high-resolution monitoring of AC133(+) tumor stem cells.

引用

页码：E692 / E701

页数：10

共 46 条

[1] Copper-64 Radiopharmaceuticals for PET Imaging of Cancer: Advances in Preclinical and Clinical Research [J].

Anderson, Carolyn J. ;

Ferdani, Riccardo .

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2009, 24 (04) :379-393

[2] Specific Elimination of CD133+ Tumor Cells with Targeted Oncolytic Measles Virus [J].

Bach, Patricia ;

Abel, Tobias ;

Hoffmann, Christopher ;

Gal, Zoltan ;

Braun, Gundula ;

Voelker, Iris ;

Ball, Claudia R. ;

Johnston, Ian C. D. ;

Lauer, Ulrich M. ;

Herold-Mende, Christel ;

Muehlebach, Michael D. ;

Glimm, Hanno ;

Buchholz, Christian J. .

CANCER RESEARCH, 2013, 73 (02) :865-874

[3] Cancer stem cells tracked [J].

Baker, Monya .

NATURE, 2012, 488 (7409) :13-14

[4] Unravelling cancer stem cell potential [J].

Beck, Benjamin ;

Blanpain, Cedric .

NATURE REVIEWS CANCER, 2013, 13 (10) :727-738

[5] CD133+ and CD133- glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles [J].

Beier, Dagmar ;

Hau, Peter ;

Proescholdt, Martin ;

Lohmeier, Annette ;

Wischhusen, Joerg ;

Oefner, Peter J. ;

Aigner, Ludwig ;

Brawanski, Alexander ;

Bogdahn, Ulrich ;

Beier, Christoph P. .

CANCER RESEARCH, 2007, 67 (09) :4010-4015

[6] Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment [J].

Bertolini, Giulia ;

Roz, Luca ;

Perego, Paola ;

Tortoreto, Monica ;

Fontanella, Enrico ;

Gatti, Laura ;

Pratesi, Graziella ;

Fabbri, Alessandra ;

Andriani, Francesca ;

Tinelli, Stella ;

Roz, Elena ;

Caserini, Roberto ;

Lo Vullo, Salvatore ;

Camerini, Tiziana ;

Mariani, Luigi ;

Delia, Domenico ;

Calabro, Elisa ;

Pastorino, Ugo ;

Sozzi, Gabriella .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (38) :16281-16286

[7] Differentiation Therapy Exerts Antitumor Effects on Stem-like Glioma Cells [J].

Campos, Benito ;

Wan, Feng ;

Farhadi, Mohammad ;

Ernst, Aurelie ;

Zeppernick, Felix ;

Tagscherer, Katrin E. ;

Ahmadi, Rezvan ;

Lohr, Jennifer ;

Dictus, Christine ;

Gdynia, Georg ;

Combs, Stephanie E. ;

Goidts, Violaine ;

Helmke, Burkhard M. ;

Eckstein, Volker ;

Roth, Wilfried ;

Beckhove, Philipp ;

Lichter, Peter ;

Unterberg, Andreas ;

Radlwimmer, Bernhard ;

Herold-Mende, Christel .

CLINICAL CANCER RESEARCH, 2010, 16 (10) :2715-2728

[8] A restricted cell population propagates glioblastoma growth after chemotherapy [J].

Chen, Jian ;

Li, Yanjiao ;

Yu, Tzong-Shiue ;

McKay, Renee M. ;

Burns, Dennis K. ;

Kernie, Steven G. ;

Parada, Luis F. .

NATURE, 2012, 488 (7412) :522-+

[9]

Chen KS, 2012, ADV EXP MED BIOL, V746, P121, DOI 10.1007/978-1-4614-3146-6_10

[10] A Hierarchy of Self-Renewing Tumor-Initiating Cell Types in Glioblastoma [J].

Chen, Ruihuan ;

Nishimura, Merry C. ;

Bumbaca, Stephanie M. ;

Kharbanda, Samir ;

Forrest, William F. ;

Kasman, Ian M. ;

Greve, Joan M. ;

Soriano, Robert H. ;

Gilmour, Laurie L. ;

Rivers, Celina Sanchez ;

Modrusan, Zora ;

Nacu, Serban ;

Guerrero, Steve ;

Edgar, Kyle A. ;

Wallin, Jeffrey J. ;

Lamszus, Katrin ;

Westphal, Manfred ;

Heim, Susanne ;

James, C. David ;

VandenBerg, Scott R. ;

Costello, Joseph F. ;

Moorefield, Scott ;

Cowdrey, Cynthia J. ;

Prados, Michael ;

Phillips, Heidi S. .

CANCER CELL, 2010, 17 (04) :362-375

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