Amplicon sequencing reveals different microbial communities in living poplar wetwood and sapwood

被引：8

作者：

Yu, Xiaoli ^{[1
]}

Hu, Xingyi ^{[2
]}

Peng, Yanjie ^{[1
]}

Wu, Zhifang ^{[1
]}

Zhang, Qilin ^{[1
]}

Li, Zhenfang ^{[2
]}

Shi, Chunmei ^{[1
]}

Du, Kebing ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Hort & Forestry Sci, Hubei Engn Technol Res Ctr Forestry Informat, Wuhan 430070, Hubei, Peoples R China

[2] Hubei Acad Forestry, Wuhan 430075, Hubei, Peoples R China

来源：

TREES-STRUCTURE AND FUNCTION | 2019年 / 33卷 / 03期

基金：

中国国家自然科学基金;

关键词：

16S rRNA; ITS; Microbial community; Populus L; Sapwood; Wetwood; BIOMASS ENZYMATIC SACCHARIFICATION; WET HEARTWOOD; DIVERSITY; BACTERIA; RHIZOSPHERE; EXTRACTION; FEATURES; TREES; WOOD; DNA;

D O I：

10.1007/s00468-019-01822-9

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

Key messageA total of 43 phyla (395 genera) of bacteria and 11 phyla (309 genera) of fungi were identified from normal sapwood (SW), wetwood (WW) and their transition zone wood (TW) in living poplar trees, with bacteria showing significantly higher diversity than fungi. Highly variable community diversities and compositions were found for bacteria but not for fungi among WW, TW and SW. Overall, 22 phyla (95 genera) bacteria and three phyla (11 genera) fungi were significantly different in relative abundance between WW and SW. Nine wood chemical properties were significantly correlated with the relative abundance of top 15 bacterial and fungal genera in all samples.AbstractWetwood represents an abnormal condition of heartwood in living poplar trees. The cause of wetwood formation is still poorly understood, but it is generally considered to involve microbial activities. To facilitate a better understanding of wetwood formation, we sequenced the 16S rRNA and ITS (Internal Transcribed Spacer) genes of distinct regions (16S V4, ITS1) to precisely identify the microbial communities in normal sapwood (SW), wetwood (WW) and their transition zone wood (TW). Nine chemical properties of the wood samples were further determined to elucidate the correlations between the microbial communities and wood characteristics. The total sequences of 16S V4 and ITS1 from WW, TW and SW were assigned to 43 bacterial phyla (395 genera) and 11 fungal phyla (309 genera), indicating a significantly higher diversity of bacteria than fungi. Among SW, TW and WW, significant variations in community diversity and composition were found for bacteria but not for fungi. WW had the highest bacterial and lowest fungal diversities, while SW exhibited the highest fungal diversity. TW generally exhibited intermediate levels between WW and SW. Between WW and SW, 22 bacterial phyla (95 genera) were significantly different in relative abundance. Nine wood chemical properties were significantly correlated with the relative abundance of top 15 bacterial and fungal genera in all wood samples.

引用

页码：851 / 865

页数：15