Improved method to quantitatively determine powerful odorant volatile thiols in wine by headspace solid-phase microextraction after derivatization

被引：39

作者：

Rodriguez-Bencomo, Juan Jose ^{[1
]}

Schneider, Remi ^{[1
]}

Lepoutre, Jean Paul ^{[1
]}

Rigou, Peggy ^{[1
]}

机构：

[1] INRA, UMR Sci Oenol, F-34060 Montpellier, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 30期

关键词：

Solid-phase microextraction; Derivatization; Volatile thiols; Wine; Aroma; Gas chromatography-mass spectrometry; CV SAUVIGNON BLANC; 3-MERCAPTOHEXYL ACETATE; PENTAFLUOROBENZYL DERIVATIVES; POLYFUNCTIONAL MERCAPTANS; AROMA; IDENTIFICATION; EXTRACTION; PRECURSORS; 4-MERCAPTO-4-METHYLPENTAN-2-ONE; COMPOUND;

D O I：

10.1016/j.chroma.2009.06.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A solid-phase microextraction (SMPE) method coupled to a gas chromatography-mass spectrometry analysis was optimized to analyze the pentafluorobenzyl bromide (PFBBr) derivatives of the volatile thiols 4-methyl-4-mercapto-2-pentanone (4MMP), 3-mercaptohexyl acetate (3MHA) and 3-mercaptohexanol (3MH) in wine. This method used deuterated analogue compounds as internal standards. It allowed us to significantly reduce the matrix effect, resulted in good repeatability for all the compounds (RDS < 9.5% for 4MMP; <6% for 3MH and <4.1% for 3MHA) with limits of detection below their odour thresholds. The method was validated using white, rose and red wines. When applied to analyze different wines, quantities closed to the odour threshold were determined. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：5640 / 5646

页数：7

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