A novel cartilage tissue construction based on artificial cells and matrix-shaping

被引：3
作者：
Liu, Yuangang [1 ,2 ]
Tang, Xiaolin [1 ]
Wang, Shibin [1 ,2 ]
Chen, Aizheng [1 ,2 ]
Wu, Wenguo [1 ,2 ]
Long, Ruimin [1 ]
机构：
[1] Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China
[2] Huaqiao Univ, Inst Pharmaceut Engn, Xiamen 361021, Peoples R China
来源：
MATERIALS LETTERS | 2015年 / 159卷
关键词：
Composite materials; Sol-gel preparation; Artificial cells; Matrix-shaping; CHONDROCYTE DIFFERENTIATION; EXTRACELLULAR-MATRIX; CULTURE; BFGF; HYDROGEL; REGENERATION; SCAFFOLD;
D O I：
10.1016/j.matlet.2015.06.068
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Cartilage tissue engineering through culturing chondrocytes in 3D porous scaffolds has potential for the repair of articular cartilage defects. To promote cell distribution in the scaffold, a novel construction method was proposed based on artificial cells and matrix-shaping. Results showed that the construct had better proliferation and ECM secretion with bFGF. Cells had the best proliferation, largest GAG and type II collagen content on day 10 in the 2D condition without bFGF, while these were enhanced by bFGF to day 15. Under 3D conditions, the highest proliferation and GAG content were on day 15 in bFGF-free medium while these two values were improved by bFGF, with the highest appearing on day 20. In conclusion, this construction method is efficient and provides a new and promising approach to cartilage regeneration. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：24 / 27
页数：4
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