Two streptokinase genes are expressed with different solubility in Escherichia coli W3110

被引：8
|
作者：
Pupo, E
Baghbaderani, BA
Lugo, V
Fernández, J
Páez, R
Torréns, I
机构：
[1] Ctr Genet Engn & Biotechnol, Biopharmaceut Dev Div, Havana, Cuba
[2] Ctr Genet Engn & Biotechnol, Div Pharmaceut, Havana, Cuba
[3] Inst Pasteur, Div Biotechnol, Teheran, Iran
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 12期
关键词：
Escherichia coli; purification; solubility; streptokinase;
D O I：
10.1023/A:1005661724123
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The streptokinase (SK) gene from S. equisimilis H46A (ATCC 12449) was cloned in E. coli W3110 under the control of the tryptophan promoter. The recombinant SK, which represented 15% of total cell protein content, was found in the soluble fraction of disrupted cells. The solubility of this SK notably differed from that of the product of the SK gene from S. equisimilis (ATCC 9542) which had been cloned in E. coli W3110 by using similar expression vector and cell growth conditions, and occurred in the form of inclusion bodies.
引用
收藏
页码：1119 / 1123
页数：5
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