Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption

被引:34
作者
Desando, Giovanna [1 ]
Bartolotti, Isabella [1 ]
Martini, Lucia [2 ]
Giavaresi, Gianluca [2 ]
Aldini, Nicolo Nicoli [2 ]
Fini, Milena [2 ]
Roffi, Alice [3 ]
Perdisa, Francesco [4 ]
Filardo, Giuseppe [3 ]
Kon, Elizaveta [5 ]
Grigolo, Brunella [1 ]
机构
[1] IRCCS Ist Ortoped Rizzoli, Lab RAMSES, I-40136 Bologna, Italy
[2] IRCCS Ist Ortoped Rizzoli, Lab Preclin & Surg Studies, I-40136 Bologna, Italy
[3] IRCCS Ist Ortoped Rizzoli, Appl & Translat Res Ctr, I-40136 Bologna, Italy
[4] IRCCS Ist Ortoped Rizzoli, Hip & Knee Replacement Dept, I-40136 Bologna, Italy
[5] Humanitas Univ, Dept Biomed Sci, Humanitas Clin & Res Ctr, I-20121 Milan, Italy
关键词
osteoarthritis; expanded adipose-derived stromal cells; adipose niche; local biodistribution; cartilage; synovial membrane; meniscus; CD-163; macrophages; MESENCHYMAL STEM-CELLS; STROMAL-VASCULAR FRACTION; NONENZYMATIC METHOD; MACROPHAGES; CHONDROCYTES; ACTIVATION; NICHE;
D O I
10.3390/ijms20112636
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evaluating cell migration after cell-based treatment is important for several disorders, including osteoarthritis (OA), as it might influence the clinical outcome. This research explores migrating expanded-adipose stromal cells (ASCs) and adipose niches after enzymatic and mechanical processes. Bilateral anterior cruciate ligament transection induced a mild grade of OA at eight weeks in adult male New Zealand rabbits. ASCs, enzymatic stromal vascular fraction (SVF), and micro fragmented adipose tissue (MFAT) were intra-articularly injected in the knee joint. Assessments of cell viability and expression of specific markers, including CD-163 wound-healing macrophages, were done. Cell migration was explored through labelling with PKH26 dye at 7 and 30 days alongside co-localization analyses for CD-146. All cells showed good viability and high percentages of CD-90 and CD-146. CD-163 was significantly higher in MFAT compared to SVF. Distinct migratory potential and time-dependent effects were observed among cell-based treatments. At day 7, both ASCs and SVF migrated towards synovium, whereas for MFAT versus cartilage, a different migration pattern was noticed at day 30. The long-term distinct cell migration of ASCs, SVF, and MFAT open interesting clinical insights on their potential use for OA treatment. Moreover, the highest expression of CD-163 in MFAT, rather than SVF, might have an important role in directly mediating cartilage tissue repair responses.
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页数:16
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