New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)

被引：6

作者：

Colliard, Caroline ^{[1
]}

Sicilia, Alessandra ^{[2
]}

Moritz, Craig ^{[3
]}

Perrin, Nicolas ^{[1
]}

Stoeck, Matthias ^{[1
]}

机构：

[1] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland

[2] Univ Palermo, Dipartimento Biol Anim, I-90123 Palermo, Italy

[3] Univ Calif Berkeley, MVZ, Dept Integrat Biol, Berkeley, CA 94720 USA

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 04期

关键词：

Bnfo viridis subgroup; cross-priming; microsatellites; mitotyping; Palearctic green toads; PCR PRIMERS; MITOCHONDRIAL; VERTEBRATE;

D O I：

10.1111/j.1755-0998.2009.02600.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

引用

页码：1138 / 1140

页数：3

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