K-19 mRNA RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer

被引：0

作者：

Suo, Jian ^{[1
]}

Wang, Quan ^{[1
]}

Jin, Hong-Juan ^{[1
]}

Li, Hong ^{[1
]}

Zhao, Hang ^{[1
]}

机构：

[1] First Hosp, Dept Gen Surg, Changchun 130021, Jilin Province, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2006年 / 12卷 / 32期

关键词：

K-19; mRNA; RT-PCR; micrometastasis; gastric cancer;

D O I：

暂无

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS: Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of gastric carcinoma. RESULTS: K19 mRNA was expressed in all tumor specimens of 30 cases; of the 126 lymph nodes, 26 were histopathologically positive (20.6%), and 42 positive (33.3%) by RT-PCR. Amplification fragments of 460 and 540 bp were shown in all the tumor tissues and metastatic lymph nodes after K19 and beta-actin RT-PCR, while only a 540 bp fragment appeared in the lymph nodes of non-tumor patients. CONCLUSION: K19 mRNA RT-PCR is sensitive and specific in testing micrometastasis in regional lymph nodes of gastric cancer, and it is superior to routine histopathology. (C) 2006 The WJG Press. All rights reserved.

引用

页码：5219 / 5222

页数：4

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